The Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase is Fragmented into 37-kDa and 16-kDa Polypeptides by Active Oxygen in the Lysates of Chloroplasts from Primary Leaves of Wheat

鲁比斯科 1,5-二磷酸核酮糖 叶绿体 加氧酶 生物化学 蛋白质亚单位 化学 生物 分子生物学 光合作用 基因
作者
H. Ishida,Y Nishimori,M. Sugisawa,Amane Makino,Tadahiko Mae
出处
期刊:Plant and Cell Physiology [Oxford University Press]
卷期号:38 (4): 471-479 被引量:155
标识
DOI:10.1093/oxfordjournals.pcp.a029191
摘要

Lysates of chloroplasts isolated from wheat (Triticum aestivum L. cv. Aoba) leaves were incubated on ice (pH 5.7) for 0 to 60 min in light (15 μmol quanta m−2 s−1), and degradation of the large subunit (LSU) of ribulose-l,5-bis-phosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) was analyzed by applying immunoblotting with site-specific antibodies against the N-terminal, internal, and C-terminal amino acid sequences of the LSU of wheat Rubisco. The most dominant product of the breakdown of the LSU and that which was first to appear was an apparent molecular mass of 37-kDa fragment containing the N-terminal region of the LSU. A 16-kDa fragment containing the C-terminal region of the LSU was concomitantly seen. This fragmentation of the LSU was inhibited in the presence of EDTA or 1,10-phenanthroline. The addition of active oxygen scavengers, catalase (for H2O2) and n-propyl gallate (for hydroxyl radical) to the lysates also inhibited the fragmentation. When the purified Rubisco from wheat leaves was exposed to a hydroxyl radical-generating system comprising H2O2, FeSO4 and ascorbic acid, the LSU was degraded in the same manner as observed in the chloroplast lysates. The results suggest that the large subunit of Rubisco was directly degraded to the 37-kDa fragment containing the N-terminal region and the 16-kDa fragment containing the C-terminal region of the LSU by active oxygen, probably the hydroxyl radical, generated in the lysates of chloroplasts.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
桐桐应助秦pale采纳,获得10
1秒前
DrY发布了新的文献求助10
2秒前
八百标兵完成签到,获得积分10
2秒前
akmdh完成签到,获得积分10
3秒前
冯先森ya发布了新的文献求助10
5秒前
量子星尘发布了新的文献求助10
6秒前
6秒前
Jonathan完成签到,获得积分10
10秒前
wsj发布了新的文献求助10
11秒前
12秒前
整齐小松鼠应助wsj采纳,获得10
16秒前
16秒前
17秒前
17秒前
20秒前
20秒前
20秒前
21秒前
Owen应助超速也文章采纳,获得10
22秒前
张雯思发布了新的文献求助10
24秒前
清爽尔安发布了新的文献求助10
24秒前
25秒前
孙燕应助幸福大白采纳,获得30
25秒前
香香应助研友_Zzrx6Z采纳,获得10
25秒前
27秒前
28秒前
28秒前
从容的柜子完成签到 ,获得积分10
29秒前
29秒前
木可发布了新的文献求助10
30秒前
清爽尔安完成签到,获得积分10
31秒前
Komorebi完成签到 ,获得积分10
31秒前
qqq发布了新的文献求助10
31秒前
所所应助独特乘云采纳,获得10
32秒前
33秒前
34秒前
小蘑菇应助发疯的草莓采纳,获得10
35秒前
小绵羊发布了新的文献求助10
36秒前
八卦巧克力完成签到,获得积分10
36秒前
lzw发布了新的文献求助10
37秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989406
求助须知:如何正确求助?哪些是违规求助? 3531522
关于积分的说明 11254187
捐赠科研通 3270174
什么是DOI,文献DOI怎么找? 1804901
邀请新用户注册赠送积分活动 882105
科研通“疑难数据库(出版商)”最低求助积分说明 809174