Ulva macroalgae within local aquaculture ponds along the estuary of Dagu River, Jiaozhou Bay, Qingdao

Green macroalgal blooms caused by Ulva species have influenced the Shandong Province for 15 consecutive years since 2007, leading to serious damage to the marine environment. Great biomasses of attached Ulva prolifera on Neopyropia aquaculture rafts in the Yellow Sea were considered as sources of blooms. However, it is still unclear whether U. prolifera could survive and settle in the Qingdao coastal environment, Shandong, thus leading to local Ulva blooms in the future. In this research, sampling of Ulva macroalgae in seven Portunus trituberculatus aquaculture ponds along the coast of Qingdao was conducted on August 21, 2019. In total, 24 samples collected from the ponds were analyzed through methods of molecular biological identification (ITS, 5S, and rps2-trnL sequences) and genetic analysis. All the aquaculture ponds contained large amounts of floating Ulva macroalgae, which consisted of three species: U. prolifera, Ulva meridionalis, and Ulva pertusa. Among these species, U. meridionalis, which is usually found in southern Japan, also causes the green tide. In addition, all Ulva macroalgae floated on the surfaces of aquaculture ponds, and were discarded into the local coastal area by aquaculturist. This research raised our awareness of the importance of controlling the spread of the green tide related macroalgae.