夜蛾
生物
兰尼定受体
生殖器鳞翅目
保幼激素
基因沉默
细胞生物学
植物
昆虫
基因
受体
生物化学
重组DNA
作者
Lin Xu,Shan‐Chi Yi,Jiu-Ying Li,Yao Tong,Cong Xie,Dongqiang Zeng,Wenwei Tang
标识
DOI:10.1021/acs.jafc.1c08083
摘要
Isoryanodane and ryanodane diterpenes have a carbon skeleton correlation in structures, and their natural product-oxidized diterpenes show antifeedant and insecticidal activities against Hemiptera and Lepidoptera. While ryanodine mainly acts on the ryanodine receptor (RyR), isoryanodane does not. In this study, we demonstrated that itol A, an isoryanodane diterpenoid, could significantly downregulate the expression level of juvenile hormone-binding protein (JHBP), which plays a vital role in JH transport. RNAi bioassay indicated that silencing the Spodoptera frugipredaJHBP (SfJHBP) gene decreased itol A activity, which confirmed the developmental phenotypic observation. Parallel reaction monitoring (PRM) further confirmed that itol A affected JHBP's expression abundance. Although JHBP is not proven as the direct or only target of itol A, we confirmed that itol A's action effect depends largely on JHBP and that JHBP is a potential target of itol A. We present foundational evidence that itol A inhibits the growth and development of Spodoptera frugiperda mainly through hijacking JHBP.
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