结直肠癌
癌症研究
细胞生长
下调和上调
体内
大肠癌小鼠模型的建立
癌症
细胞
医学
生物
内科学
基因
生物化学
遗传学
生物技术
作者
Zhanke He,Jun-bo Yang,Chuyang Sui,Penghao Zhang,Ting Wang,Tingyu Mou,Kai Sun,Yanan Wang,Zhijun Xu,Guoxin Li,Haijun Deng,Jiaolong Shi,Baoxiong Zhuang
标识
DOI:10.1016/j.abb.2022.109216
摘要
FAM98A is a microtubule-associated protein involved in cell proliferation and migration, and is frequently dysregulated in epithelial cancers. But its role in the development of colorectal cancer (CRC) cancer remains unknown.Immunohistochemical analysis was performed to examine the expression of FAM98A in CRC samples. We also investigated the effects of abnormal expression on the biological behavior of colorectal cancer cells both in vitro and in vivo. Immunoblotting and immunoprecipitation were used to screen FAM98A-related signalling pathways and downstream factors.FAM98A was upregulated in CRC tissues and CRC cell lines. Overexpression of FAM98A promoted cell proliferation and recovered 5-FU suppressed CRC cell proliferation both in vitro and in vivo. In addition, the Enhanced expression of FAM98A inhibited ferroptosis in CRC cells by activating the translation of xCT in stress granules (SGs). Furthermore, we identified that metformin could reverse FAM98A-mediated 5-FU resistance in CRC cells.Our results for the first time indicate that FAM98A plays a critical role in promoting CRC progression, which provides a novel target for clinical drug resistance of colorectal cancer. And metformin may sensitize 5-FU in the treatment of colorectal cancer.
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