Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs

盲肠 回肠 短链脂肪酸 空肠 胃肠道 内科学 生物 甘油三酯 丁酸盐 内分泌学 盲肠 生物化学 胆固醇 医学 发酵
作者
Barbara U. Metzler‐Zebeli,Jutamat Klinsoda,Julia Vötterl,Suchitra Sharma,Simone Koger,Arife Sener-Aydemir
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:99 (6) 被引量:10
标识
DOI:10.1093/jas/skab117
摘要

Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 ± 2.3 kg) fed diets containing either phytase (500 phytase units/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P < 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P < 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P < 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P < 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P < 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P < 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P < 0.05). Pearson's correlations (|r| > 0.35; P < 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
胖子完成签到,获得积分10
1秒前
3秒前
Hezhiyong完成签到,获得积分10
4秒前
秋听寒完成签到,获得积分10
5秒前
李健应助斯奈克采纳,获得20
5秒前
6秒前
大模型应助酷炫的不悔采纳,获得10
6秒前
超级的路人完成签到,获得积分10
7秒前
LilyChen完成签到 ,获得积分10
7秒前
7秒前
光亮平蝶发布了新的文献求助10
8秒前
摆不烂发布了新的文献求助10
8秒前
诸葛不亮发布了新的文献求助10
8秒前
爱看文献的小羽毛完成签到,获得积分10
10秒前
酷炫的不悔完成签到,获得积分10
12秒前
弗一昂发布了新的文献求助10
12秒前
丘比特应助傅三毒采纳,获得10
13秒前
Yan完成签到,获得积分10
14秒前
Ava应助鹰击长空采纳,获得10
14秒前
孤独的大灰狼完成签到 ,获得积分10
14秒前
cannon8应助高贵的夜南采纳,获得20
14秒前
CipherSage应助Riverside采纳,获得10
15秒前
孝顺的青枫完成签到,获得积分10
16秒前
科研通AI2S应助TsutsumiRyuu采纳,获得10
18秒前
妖怪大大应助kaixin采纳,获得10
19秒前
19秒前
英姑应助佩奇采纳,获得10
21秒前
21秒前
ironsilica发布了新的文献求助10
22秒前
研友_祝鬼神完成签到,获得积分10
22秒前
摆不烂完成签到,获得积分10
22秒前
Cody发布了新的文献求助10
23秒前
Orange应助飞天817采纳,获得10
24秒前
尊敬又晴完成签到,获得积分10
24秒前
Gilana发布了新的文献求助10
25秒前
ktk完成签到,获得积分10
27秒前
xiao完成签到,获得积分10
27秒前
NexusExplorer应助5165asd采纳,获得10
29秒前
顺心的芹菜完成签到,获得积分10
30秒前
哥哥喜欢格格完成签到 ,获得积分10
30秒前
高分求助中
Evolution 3rd edition 1500
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
Ribozymes and aptamers in the RNA world, and in synthetic biology 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3180952
求助须知:如何正确求助?哪些是违规求助? 2831093
关于积分的说明 7983278
捐赠科研通 2493095
什么是DOI,文献DOI怎么找? 1329977
科研通“疑难数据库(出版商)”最低求助积分说明 635872
版权声明 602954