亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs

盲肠 回肠 短链脂肪酸 空肠 胃肠道 内科学 生物 甘油三酯 丁酸盐 内分泌学 盲肠 生物化学 胆固醇 医学 发酵
作者
Barbara U. Metzler‐Zebeli,Jutamat Klinsoda,Julia Vötterl,Suchitra Sharma,Simone Koger,Arife Sener-Aydemir
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:99 (6) 被引量:10
标识
DOI:10.1093/jas/skab117
摘要

Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 ± 2.3 kg) fed diets containing either phytase (500 phytase units/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P < 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P < 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P < 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P < 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P < 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P < 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P < 0.05). Pearson's correlations (|r| > 0.35; P < 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
完美世界应助ShenghuiH采纳,获得10
27秒前
28秒前
35秒前
49秒前
Little青完成签到 ,获得积分10
50秒前
ShenghuiH发布了新的文献求助10
56秒前
ShenghuiH完成签到,获得积分10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
smallfish发布了新的文献求助10
1分钟前
lazysheep完成签到,获得积分10
1分钟前
1分钟前
Anonyme发布了新的文献求助10
1分钟前
向阳葵完成签到 ,获得积分10
1分钟前
完美世界应助smallfish采纳,获得10
1分钟前
Doctor_jie完成签到 ,获得积分10
1分钟前
1分钟前
2分钟前
2分钟前
2分钟前
科研通AI2S应助风趣的忆南采纳,获得10
2分钟前
morena发布了新的文献求助10
2分钟前
心脏杀手发布了新的文献求助10
2分钟前
无语的诗柳完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
心脏杀手完成签到,获得积分10
2分钟前
Anonyme完成签到,获得积分20
2分钟前
Anonyme发布了新的文献求助10
3分钟前
小蘑菇应助科研通管家采纳,获得10
3分钟前
WXR完成签到 ,获得积分10
3分钟前
只如初完成签到,获得积分10
3分钟前
充电宝应助morena采纳,获得10
3分钟前
3分钟前
4分钟前
lige完成签到 ,获得积分10
4分钟前
4分钟前
4分钟前
4分钟前
4分钟前
西吴完成签到 ,获得积分10
4分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3200737
求助须知:如何正确求助?哪些是违规求助? 2850476
关于积分的说明 8072154
捐赠科研通 2514239
什么是DOI,文献DOI怎么找? 1346972
科研通“疑难数据库(出版商)”最低求助积分说明 640303
邀请新用户注册赠送积分活动 610411