已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs

盲肠 回肠 短链脂肪酸 空肠 胃肠道 内科学 生物 甘油三酯 丁酸盐 内分泌学 盲肠 生物化学 胆固醇 医学 发酵
作者
Barbara U. Metzler‐Zebeli,Jutamat Klinsoda,Julia Vötterl,Suchitra Sharma,Simone Koger,Arife Sener-Aydemir
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:99 (6) 被引量:10
标识
DOI:10.1093/jas/skab117
摘要

Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 ± 2.3 kg) fed diets containing either phytase (500 phytase units/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P < 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P < 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P < 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P < 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P < 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P < 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P < 0.05). Pearson's correlations (|r| > 0.35; P < 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
鹏程万里完成签到,获得积分10
2秒前
6秒前
Akim应助小章采纳,获得10
7秒前
9秒前
机灵柚子发布了新的文献求助20
11秒前
科研通AI2S应助epitome采纳,获得10
12秒前
LS-GENIUS发布了新的文献求助10
14秒前
15秒前
一念春风发布了新的文献求助100
16秒前
想发sci发布了新的文献求助30
17秒前
19秒前
我是老大应助ddfighting采纳,获得10
23秒前
sissiarno应助咸鱼想翻身采纳,获得60
26秒前
Karen331完成签到 ,获得积分10
28秒前
慕青应助King16采纳,获得10
29秒前
31秒前
义气雍发布了新的文献求助10
35秒前
qqqq完成签到 ,获得积分10
35秒前
研友_VZG7GZ应助言隐采纳,获得30
38秒前
40秒前
41秒前
41秒前
可靠的之瑶完成签到,获得积分20
43秒前
科研通AI2S应助机灵柚子采纳,获得10
43秒前
高高一一发布了新的文献求助30
45秒前
45秒前
46秒前
vvvvvvvvvk发布了新的文献求助30
46秒前
酷波er应助背后访风采纳,获得10
46秒前
英姑应助小章采纳,获得10
47秒前
pyc关闭了pyc文献求助
48秒前
阔达白筠发布了新的文献求助10
49秒前
King16发布了新的文献求助10
49秒前
51秒前
51秒前
51秒前
51秒前
Baekhyun发布了新的文献求助10
54秒前
ss完成签到,获得积分10
56秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3186420
求助须知:如何正确求助?哪些是违规求助? 2836756
关于积分的说明 8010933
捐赠科研通 2499098
什么是DOI,文献DOI怎么找? 1334065
科研通“疑难数据库(出版商)”最低求助积分说明 637023
邀请新用户注册赠送积分活动 604986