Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs

盲肠 回肠 短链脂肪酸 空肠 胃肠道 内科学 生物 甘油三酯 丁酸盐 内分泌学 盲肠 生物化学 胆固醇 医学 发酵
作者
Barbara U. Metzler‐Zebeli,Jutamat Klinsoda,Julia Vötterl,Suchitra Sharma,Simone Koger,Arife Sener-Aydemir
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:99 (6) 被引量:10
标识
DOI:10.1093/jas/skab117
摘要

Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 ± 2.3 kg) fed diets containing either phytase (500 phytase units/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P < 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P < 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P < 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P < 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P < 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P < 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P < 0.05). Pearson's correlations (|r| > 0.35; P < 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
1秒前
白色热干面完成签到,获得积分10
1秒前
彭于晏女友完成签到,获得积分10
1秒前
情怀应助科研人采纳,获得10
2秒前
露亮完成签到,获得积分10
3秒前
思源应助温暖听莲采纳,获得10
4秒前
xxx发布了新的文献求助10
4秒前
希望天下0贩的0应助Liqy采纳,获得10
6秒前
陈木子发布了新的文献求助10
6秒前
6秒前
柟枫完成签到,获得积分10
7秒前
乐乐应助刘亦菲采纳,获得30
7秒前
9秒前
顾矜应助guoll采纳,获得10
9秒前
奔跑的兔子完成签到,获得积分10
11秒前
ymym发布了新的文献求助10
11秒前
陈疾坎完成签到 ,获得积分10
13秒前
13秒前
英俊的铭应助fff采纳,获得10
14秒前
科研人发布了新的文献求助10
14秒前
14秒前
所所应助xxx采纳,获得10
14秒前
15秒前
辛勤的青易完成签到,获得积分20
15秒前
15秒前
15秒前
飞秋完成签到,获得积分10
15秒前
小小发布了新的文献求助10
16秒前
秋酿完成签到 ,获得积分10
17秒前
科研完成签到,获得积分10
18秒前
万能图书馆应助ymym采纳,获得10
18秒前
NEWBIE发布了新的文献求助10
18秒前
zj发布了新的文献求助10
19秒前
Liqy发布了新的文献求助10
19秒前
飞秋发布了新的文献求助10
19秒前
材料人完成签到,获得积分10
21秒前
simple应助cc采纳,获得10
21秒前
淡然的寻冬完成签到 ,获得积分10
22秒前
达不溜踢踢完成签到 ,获得积分10
22秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Bioinspired single-shot polarization photodetector based on four-directional grating arrays capped perovskite single-crystal thin film 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3188539
求助须知:如何正确求助?哪些是违规求助? 2837962
关于积分的说明 8018201
捐赠科研通 2500699
什么是DOI,文献DOI怎么找? 1335163
科研通“疑难数据库(出版商)”最低求助积分说明 637466
邀请新用户注册赠送积分活动 605506