已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Short-, medium-, and long-chain fatty acid profiles and signaling is responsive to dietary phytase and lactic acid treatment of cereals along the gastrointestinal tract of growing pigs

盲肠 回肠 短链脂肪酸 空肠 胃肠道 内科学 生物 甘油三酯 丁酸盐 内分泌学 盲肠 生物化学 胆固醇 医学 发酵
作者
Barbara U. Metzler‐Zebeli,Jutamat Klinsoda,Julia Vötterl,Suchitra Sharma,Simone Koger,Arife Sener-Aydemir
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:99 (6) 被引量:10
标识
DOI:10.1093/jas/skab117
摘要

Dietary and microbially derived fatty acids (FA) play important roles in gut mucosal inflammatory signaling, barrier function, and oxidative stress response. Nevertheless, little information is available about gastrointestinal FA profiles and receptor distribution in pigs, especially for long-chain FA (LCFA). Therefore, the present pilot study aimed to (1) investigate the gastrointestinal FA profiles; (2) link the luminal FA profiles to the mucosal expression of genes related to FA sensing and signaling; and (3) assess potential dietary effects on gut and systemic lipid metabolism in pigs. Gut, liver, and serum samples were obtained from barrows (13.1 ± 2.3 kg) fed diets containing either phytase (500 phytase units/kg diet) or cereals treated with 2.5% lactic acid (LA; n = 8/diet) for 18 d. Results showed gut regional and diet-related differences in luminal FA profiles and mucosal receptor expression, whereas diet little affected hepatic expression levels and serum lipids. Short-chain fatty acids (SCFA) increased from stomach, jejunum, and ileum to the cecum (P < 0.05), whereas LCFA were higher in stomach, cecum, and colon than in jejunum and ileum (P < 0.05). LA-treated cereals enhanced cecal acetate and butyrate, whereas phytase and LA treated cereals decreased the LCFA by 35.9% and 14.4%, respectively (P < 0.05). Gut regional differences suggested stronger signaling via FFAR1 expression in the ileum, and via FFAR2, FFAR4, and HCAR1 expression in cecum and colon (P < 0.05). Expression of AMPK, FASN, PPARG, SREBP1, and SREBP2 was higher in the cecum and colon compared with the small intestine (P < 0.05), with stronger sensing via FASN and SREBP2. Phytase decreased expression of FFAR2 and FFAR4, whereas it increased that of FFAR3 and MCT1 in the cecum (P < 0.05). LA-treated cereals raised cecal expression of FFAR3 and HCAR1 (P < 0.05). Pearson's correlations (|r| > 0.35; P < 0.05) supported that FA receptor- and nuclear transcription factor-dependent pathways were involved in the mucosal regulation of gut incretin expression but differed across gut regions. In conclusion, results support regional differences in SCFA, lactate and LCFA sensing and absorption capacities in the small and large intestines of pigs. Effects of phytase and the LA-treated cereals on intestinal FA levels and signaling can be explained by differences in nutrient flows (e.g., phosphorus and carbohydrate fractions). This overview provides a solid basis for future intestinal FA sensing in pigs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Sunflower完成签到,获得积分10
2秒前
4秒前
123完成签到 ,获得积分10
6秒前
桃子e完成签到 ,获得积分10
7秒前
bkagyin应助lan采纳,获得10
8秒前
8秒前
方方别方完成签到 ,获得积分10
11秒前
12秒前
明钟达完成签到,获得积分10
12秒前
lan完成签到,获得积分10
14秒前
超帅慕晴完成签到,获得积分10
15秒前
斯文的难破完成签到 ,获得积分10
16秒前
李昕123完成签到 ,获得积分10
17秒前
19秒前
糖加三勺完成签到 ,获得积分10
20秒前
kk完成签到 ,获得积分10
22秒前
α(阿尔法)完成签到 ,获得积分10
23秒前
24秒前
minmi发布了新的文献求助10
29秒前
追寻哲瀚完成签到 ,获得积分10
29秒前
如约而至完成签到 ,获得积分10
31秒前
34秒前
Ring完成签到 ,获得积分10
36秒前
唐新惠完成签到 ,获得积分10
38秒前
pegasus0802完成签到,获得积分10
38秒前
收拾完完成签到 ,获得积分10
39秒前
留下记忆完成签到 ,获得积分10
46秒前
48秒前
49秒前
只如初完成签到,获得积分10
49秒前
minmi完成签到,获得积分20
51秒前
闲庭完成签到 ,获得积分10
51秒前
与月同行完成签到,获得积分10
52秒前
kjding发布了新的文献求助10
52秒前
勿昂完成签到 ,获得积分10
53秒前
WYJ完成签到,获得积分10
55秒前
Lucas应助科研通管家采纳,获得30
55秒前
科研通AI2S应助科研通管家采纳,获得10
55秒前
科研通AI2S应助科研通管家采纳,获得10
55秒前
55秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
Idoxuridine 260
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196557
求助须知:如何正确求助?哪些是违规求助? 2845245
关于积分的说明 8053685
捐赠科研通 2509895
什么是DOI,文献DOI怎么找? 1342090
科研通“疑难数据库(出版商)”最低求助积分说明 639304
邀请新用户注册赠送积分活动 608595