化学
吞吐量
电化学发光
高通量筛选
色谱法
检出限
免疫分析
再现性
分子生物学
组蛋白
计算机科学
DNA
生物
生物化学
遗传学
电信
无线
抗体
作者
Chang Liu,Yu Qie,Lixia Zhao,Minjie Li,Liang‐Hong Guo
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2021-09-30
卷期号:6 (10): 3724-3732
被引量:11
标识
DOI:10.1021/acssensors.1c01502
摘要
Sensitive detection of phosphorylated histone H2AX (γH2AX) in cells as a biomarker of DNA double-strand breaks has great significance in the field of molecular toxicology and life science research. However, current γH2AX detection methods require labor- and time-consuming steps. Here, for the first time, we designed a simple electrochemiluminescence (ECL) immunoassay integrated with a microplate-based sensor array to realize sensitive and high-throughput detection of γH2AX in cell lysates. Under the optimized conditions, this ECL immunosensor array could linearly respond to γH2AX concentrations in the range from 2 × 102 to 1 × 105 pg/mL. In addition, our approach possessed excellent specificity and satisfactory reproducibility, and its practicality was verified in real cell lysates. The whole process including instrumental and manual operation was completed in no more than 3 h. This study provides a convenient and rapid alternative method for the sensitive quantification of γH2AX, which shows promising application in high-throughput screening of genotoxic chemicals and drug candidates.
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