免疫印迹
合成代谢
椎间盘
细胞外基质
化学
环状RNA
转化生长因子
体内
软骨细胞
软骨
细胞生物学
变性(医学)
下调和上调
医学
阿格里坎
分子生物学
病理
解剖
基因
生物
体外
生物化学
骨关节炎
关节软骨
替代医学
生物技术
作者
Bo Hu,Liang Xiao,Chong Wang,Chen Liu,Yu Zhang,Baiyang Ding,Daokuan Gao,Yan-qing Lu,Haixia Xu
出处
期刊:Bone
[Elsevier]
日期:2022-01-01
卷期号:154: 116185-116185
被引量:11
标识
DOI:10.1016/j.bone.2021.116185
摘要
Circular RNAs (circRNAs) participate in the progression of many diseases, but knowledge on the role of circRNAs in intervertebral disc degeneration (IDD) is limited. In this study, we discovered the characteristics of a new circRNA (circ_0022382) in human endplate chondrocytes. Currently, real-time quantitative polymerase chain reaction (RT-qPCR) showed that the relative expression level of circ_0022382 was significantly lower under intermittent cyclic tension stimulation than in the control group. circ_0022382, miR-4726-5p and Transforming growth factor 3 (TGF-β3) were evaluated by RT-qPCR, Western Blot and immunofluorescence assay. Additionally, the role and mechanism of circ_0022382 in vivo were also consistent in the rat model. Furthermore, Intermittent cyclic mechanical tension can cause degeneration of endplate chondrocytes. The tension-sensitive circRNA_0022382 was decreased, and we found that circRNA_0022382 promoted morphology of endplate chondrocytes by sponge-binding miR-4726-5p down-regulation of target gene the TGF-β3 expression, thereby alleviating IDD. In a rat model of acupuncture, intervertebral disc injection of circ_0022382 relieved the progression of IDD in vivo. In conclusion, the circ_0022382/miR-4726-5p/TGF-β3 axis plays a key role in the anabolism and catabolism of the endplate chondrocyte extracellular matrix (ECM). It is suggested that circ_0022382 may provide a new approach for the prevention and treatment of IDD.
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