电化学发光
检出限
鲁米诺
过氧化氢
化学发光
胶体金
重复性
线性范围
化学
纳米技术
材料科学
色谱法
组合化学
纳米颗粒
有机化学
作者
Xiaojie Shi,Huimin Liu,Mei Zhang,Fengzhen Yang,Jiansen Li,Yemin Guo
标识
DOI:10.1016/j.snb.2021.130663
摘要
An ultrasensitive aptasensor based on luminol-hydrogen peroxide (H2O2) electrochemiluminescence (ECL) system was proposed for determination of profenofos. AuNPs decorated with MWCNTs ([email protected]) were used to provide a favorable microenvironment for luminol loading and improve the ECL signal due to their good biological activity, excellent electrical conductivity and high specific surface area. Gold and silver core-shell nanoparticles ([email protected]) could act as effective catalyzer to accelerate H2O2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL signal again. Under optimal conditions, the proposed ECL aptasensor obtained an extremely low detection limit of 5.32 fg/mL (S/N = 3) with a good detection range from 100 fg/mL to 1 μg/mL. Moreover, the proposed aptasensor had excellent specificity, outstanding repeatability and favorable stability. Finally, the proposed ECL aptasensor was used for the detection of profenofos in real samples and the recoveries ranged from 94% to 102%. This strategy could provide a good reference for developing effective aptasensors for detection of other small molecules and toxins.
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