奇异变形杆菌
寻常变形杆菌
变形杆菌
塔克曼
微生物学
生物
实时聚合酶链反应
细菌
大肠杆菌
金黄色葡萄球菌
基因
遗传学
作者
Ruifu Yang,Guoyang Xu,Xiaoyou Wang,ZHICHU QING,Lizhi Fu
出处
期刊:Polish Journal of Microbiology
[Exeley Inc]
日期:2020-08-25
卷期号:69 (3): 293-300
被引量:3
标识
DOI:10.33073/pjm-2020-032
摘要
Abstract Proteus species are common opportunistic bacteria and foodborne pathogens. The proper detection of Proteus can effectively reduce the occurrence of food-borne public health events. Proteus mirabilis and Proteus vulgaris are the two most important pathogens in the Proteus genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish P. mirabilis and P. vulgaris in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of P. mirabilis and P. vulgaris in pure bacterial cultures were 6.08 × 10 2 colony forming units (CFU)/ml and 4.46 × 10 2 CFU/ml, respectively. Additionally, the minimum detectable number of P. mirabilis and P. vulgaris in meat and milk was 10 3 CFU/g. In addition, the method can be used to distinguish between strains of P. mirabilis and P. vulgaris within two hours. Overall, it is a sensitive, easy-to-use, and practical test for the identification and classification of Proteus in food.
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