Chemical Tools for Endogenous Protein Labeling and Profiling

背景(考古学) 计算生物学 化学基因学 生物 化学生物学 功能(生物学) 蛋白质组学 生物化学 仿形(计算机编程) 小分子 蛋白质组 细胞生物学 计算机科学 基因 操作系统 古生物学
作者
Kazuki Shiraiwa,Rong Cheng,Hiroshi Nonaka,Tomonori Tamura,Itaru Hamachi
出处
期刊:Cell chemical biology [Elsevier BV]
卷期号:27 (8): 970-985 被引量:65
标识
DOI:10.1016/j.chembiol.2020.06.016
摘要

Protein analysis under biological conditions is now regarded as indispensable for understanding the structure and function of proteins, in addition to in vitro studies using purified target proteins. Because there are many molecules other than the protein-of-interest (POI) under live cell conditions, selective labeling of a POI is critical to distinguish the POI from other proteins for precise analysis. Protein labeling strategies utilizing genetically encoded tags have been used in POI modification in the complex environment of live cells. However, genetic manipulation may often induce overexpression of the POI and/or perturb the cellular context, resulting in unexpected artifacts in the protein analysis. Alternatively, recent progress in chemical biology has produced two major chemical approaches for analyzing endogenous proteins under native conditions. In this review, we summarize these techniques that utilize either protein-selective chemical labeling or proteome-directed chemical modification. Protein analysis under biological conditions is now regarded as indispensable for understanding the structure and function of proteins, in addition to in vitro studies using purified target proteins. Because there are many molecules other than the protein-of-interest (POI) under live cell conditions, selective labeling of a POI is critical to distinguish the POI from other proteins for precise analysis. Protein labeling strategies utilizing genetically encoded tags have been used in POI modification in the complex environment of live cells. However, genetic manipulation may often induce overexpression of the POI and/or perturb the cellular context, resulting in unexpected artifacts in the protein analysis. Alternatively, recent progress in chemical biology has produced two major chemical approaches for analyzing endogenous proteins under native conditions. In this review, we summarize these techniques that utilize either protein-selective chemical labeling or proteome-directed chemical modification.
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