时尚
死亡域
异丙肾上腺素
肌钙蛋白复合物
心肌梗塞
医学
半胱氨酸蛋白酶8
肿瘤坏死因子α
小RNA
化学
内科学
癌症研究
细胞凋亡
内分泌学
药理学
肌钙蛋白I
半胱氨酸蛋白酶3
程序性细胞死亡
半胱氨酸蛋白酶
生物化学
刺激
基因
作者
Mai A. Zaafan,Amr M. Abdelhamid
出处
期刊:DOAJ: Directory of Open Access Journals - DOAJ
日期:2021-01-01
卷期号:25 (2): 837-844
被引量:18
标识
DOI:10.26355/eurrev_202101_24648
摘要
The current study investigates the effect of the innovative phosphorothioate modified backbone locked nucleic acid (LNA) of microRNA-103 (miR-103) specifically designed for systemic delivery in the silencing of miR-103 in experimentally induced myocardial infarction (MI). MicroRNA-103 is a small non-coding RNA which regulates Fas-associated protein with death domain (FADD) gene expression, which is a negative regulator for necroptosis occurs during the progression of MI.Experimental male mice were allocated into three groups; the first group received normal saline, the second group was injected with isoprenaline and served as the infarcted control, while the third group was treated with LNA miR-103 power inhibitor before isoprenaline injection. Blood and heart samples were used for biochemical analysis of miR-103, FADD, receptor-interacting protein kinase (RIPK), nuclear factor-κβ, tumor necrosis factor-α, interleukin-6, troponin-I and creatine kinase-MB (CK-MB) as well as the histological examination of heart tissue.The treated mice showed marked improvement in the troponin-I and CK-MB levels with almost normal histological structure of heart tissue. Significant inhibition of miR-103 accompanied by increased FADD expression and markedly decreased expression of the other biomarkers were observed in the hearts of the treated mice.LNA miR-103 inhibitor is a potent cardioprotective agent and can be a promising treatment against MI through targeting FADD/RIPK pathway.
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