小胶质细胞
人脑
生物
神经科学
转录组
胚胎干细胞
阿尔茨海默病
神经退行性变
疾病
干细胞
基因
免疫学
医学
细胞生物学
病理
基因表达
遗传学
炎症
作者
Renzo Mancuso,Johanna Van Daele,Nicola Fattorelli,Leen Wolfs,Sriram Balusu,Oliver T. Burton,Adrian Liston,Annerieke Sierksma,Yannick Fourne,Suresh Poovathingal,Amaia M. Arranz,Carlo Sala Frigerio,Christel Claes,Lutgarde Serneels,Tom Theys,V. Hugh Perry,Cathérine Verfaillie,Mark Fiers,Bart De Strooper
标识
DOI:10.1038/s41593-019-0525-x
摘要
Although genetics highlights the role of microglia in Alzheimer’s disease, one-third of putative Alzheimer’s disease risk genes lack adequate mouse orthologs. Here we successfully engraft human microglia derived from embryonic stem cells in the mouse brain. The cells recapitulate transcriptionally human primary microglia ex vivo and show expression of human-specific Alzheimer’s disease risk genes. Oligomeric amyloid-β induces a divergent response in human versus mouse microglia. This model can be used to study the role of microglia in neurological diseases. Human stem cell-derived microglia integrate into mouse brain, displaying transcriptome signatures of microglia directly isolated from human brain and providing a chimeric model to study human-specific aspects of Alzheimer’s disease and other brain diseases.
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