Advances in the chemical constituents and chemical analysis of Ginkgo biloba leaf, extract, and phytopharmaceuticals

Ginkgo biloba leaf (GBL) is an important botanical drug that can be used for treating many diseases. This review summarizes the reported chemical constituents from GBL or Ginkgo biloba extract (GBE) to date, as well as the recent advances in the extraction, purification, qualitative and quantitative analysis methods (from 2015 to 2020). To date, about 110 flavonoids have been reported to have unambiguous structures, including flavonol and its glycosides, flavone and its glycosides, flavanone and its glycosides, isoflavone and its glycosides, flavan-3-ols, bioflavonoids, and biginkgosides. In recent years, in addition to new flavonoids, new terpenoids and lignan have been also isolated from GBL. Further, several extraction and purification methods have been described and compared. Quantitative analysis of the constituents have been mainly carried out by high-performance liquid chromatography with different detector methods. Many studies have focused on variations of compounds contents in GBL from different regions, tree ages, or collection times, which provide references for the selection of GBL. Liquid chromatography-mass spectrometry coupled with activity assay methods were used to on-line screen the bioactive compounds from GBL or its phytopharmaceuticals. The application of other analytical technologies such as MS imaging, supercritical fluid chromatography, capillary electrophoresis, quantitative nuclear magnetic resonance, and spectroscopy, has also been discussed. This review of the chemical constituents and analytical methods of Ginkgo will provide a reference for the research on the quality control and discovery of effective constituents for GBL and its related phytopharmaceuticals.