伊米奎莫德
银屑病
FOXP3型
免疫系统
医学
免疫学
炎症
发病机制
流式细胞术
作者
Chong Won Choi,Bo Ri Kim,Seoyun Yang,Yejin Kim,Jae Seung Kang,Sang Woong Youn
标识
DOI:10.1016/j.jdermsci.2020.04.008
摘要
The imiquimod-induced psoriasis-like mouse model is a well-established psoriasis animal model. Thus far, studies have focused on the acute phase of psoriasis and the role of Th17 cells and the IL-23/IL-17 axis [ [1] Terhorst D. Chelbi R. Wohn C. Malosse C. Tamoutounour S. Jorquera A. Bajenoff M. Dalod M. Malissen B. Henri S. Dynamics and transcriptomics of skin dendritic cells and macrophages in an imiquimod-induced, biphasic mouse model of psoriasis. J. Immunol. 2015; 195: 4953-4961 Crossref PubMed Scopus (63) Google Scholar ]. However, those investigating the role of Treg cells in this mouse model pathogenesis are limited. In this study, imiquimod was applied longer than usual previous studies, and the role of Treg cells in imiquimod-induced psoriasis-like mouse model pathogenesis was investigated. The 7-day imiquimod application (a topical dose of 62.5 mg of 5% imiquimod cream (Aldara®; 3 M Pharmaceuticals, UK), applied to a shaved area of the back and ears) revealed that psoriasis-like inflammation severity increased with the initiation of imiquimod application, but reached a plateau after 5 days of application (Fig. 1a). This plateau suggests the existence of immune regulatory mechanisms in the mouse model. Given that Treg cells play a major role in regulating the immune response, we investigated Treg cell changes induced by long term imiquimod application. Flow cytometry using the draining lymph nodes harvested on Day 8 revealed an increase in CD4+Foxp3+ cells in draining LN post imiquimod application (Fig. 1b). In addition, the immunohistochemical analysis for Treg cells using back skin (epidermis and dermis) harvested on Day 8 showed an increased number of Treg cells in imiquimod applied mouse (P = 0.006) (Fig. S1).
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