青霉素酰胺酶
化学
青霉素
酶
细菌
抗生素
生物化学
组合化学
微生物学
生物
遗传学
作者
Luis E. Cobos‐Puc,Raúl Rodríguez‐Herrera,Juan C. Cano-Cabrera,Hilda Aguayo‐Morales,Sonia Yesenia Silva‐Belmares,Adriana C. Flores‐Gallegos,José L. Martínez‐Hernández
出处
期刊:Current Pharmaceutical Biotechnology
[Bentham Science]
日期:2019-11-12
卷期号:21 (4): 287-297
被引量:14
标识
DOI:10.2174/1389201020666191111151642
摘要
β-lactam antibiotics are the most used worldwide for the treatment of bacterial infections. The consumption of these classes of drugs is high, and it is increasing around the world. To date, the best way to produce them is using penicillin G Acylase (PGA) as a biocatalyst.This manuscript offers an overview of the most recent advances in the current tools to improve the activity of the PGA and its pharmaceutical application.Several microorganisms produce PGA, but some bacterial strains represent the primary source of this enzyme. The activity of bacterial PGA depends on its adequate expression and carbon or nitrogen source, as well as a specific pH or temperature depending on the nature of the PGA. Additionally, the PGA activity can be enhanced by immobilizing it to a solid support to recycle it for a prolonged time. Likewise, PGAs more stable and with higher activity are obtained from bacterial hosts genetically modified.PGA is used to produce b-lactam antibiotics. However, this enzyme has pharmaceutical potential to be used to obtain critical molecules for the synthesis of anti-tumor, antiplatelet, antiemetic, antidepressive, anti-retroviral, antioxidant, and antimutagenic drugs.
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