Ratiometric fluorescence sensor for organophosphorus pesticide detection based on opposite responses of two fluorescence reagents to MnO2 nanosheets

荧光 化学 检出限 试剂 氯化丹酯 乙酰胆碱酯酶 线性范围 分解 光化学 色谱法 质谱法 有机化学 物理 量子力学 衍生化
作者
Tiantian Yao,Anran Liu,Yong Liu,Min Wei,Wei Wei,Songqin Liu
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:145: 111705-111705 被引量:98
标识
DOI:10.1016/j.bios.2019.111705
摘要

The detection of organophosphorus pesticides (OPs) has received considerable attention for their great harm to human beings. Herein, a novel ratiometric fluorescence biosensor was constructed for the determination of OPs by using Scopoletin (SC) and Amplex Red (AR) as probe pairs that have opposite responses to MnO2 nanosheets (MnO2 NS). MnO2 NS possess peroxidase-like catalytic activity, which could quench the fluorescence of SC as well as enhance the fluorescence of the non-fluorescent substance AR by oxidation. In the absence of OPs, acetylcholinesterase (AChE) hydrolyzed acetylcholine chloride (ATCh) into choline (TCh) and acetate. TCh led the decomposition of MnO2 NS to manganese ions (Mn2+), increasing signal of SC and decreasing signal of AR. In the presence of OPs, the activity of AChE was inhibited and the decomposition of MnO2 NS was hindered, therefore the fluorescence intensity of SC was weak and the fluorescence intensity of AR had an obvious increase. Moreover, under the optimal conditions, the ratio of fluorescence intensity response recorded on the AR/SC increases with increasing the concentration of DDVP. The method has wider linear range of 5.0 pg/mL ∼500 ng/mL with a detection limit of 1.6 pg/mL, which is superior to previously reported methods. This strategy has also been applied to a visual observation based on the color change of the solution under UV light.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
所所应助zjz采纳,获得30
2秒前
2秒前
小吴同学发布了新的文献求助10
3秒前
量子星尘发布了新的文献求助10
3秒前
3秒前
4秒前
shaocat完成签到 ,获得积分10
4秒前
风中的眼神完成签到,获得积分10
4秒前
CAOHOU应助奋斗水香采纳,获得10
5秒前
俊逸梦蕊完成签到,获得积分10
5秒前
6秒前
典雅牛青关注了科研通微信公众号
6秒前
Xinxxx发布了新的文献求助10
6秒前
illusion完成签到,获得积分10
7秒前
wanci应助王冉冉采纳,获得30
8秒前
树小夏发布了新的文献求助10
9秒前
小吴同学完成签到,获得积分10
9秒前
赘婿应助kk_yang采纳,获得10
10秒前
成就伟祺关注了科研通微信公众号
11秒前
能干的语芙完成签到 ,获得积分10
11秒前
无欲无求傻傻完成签到,获得积分10
11秒前
11秒前
11秒前
尊敬寒松完成签到 ,获得积分10
11秒前
糊涂的麦片完成签到,获得积分10
11秒前
12秒前
12秒前
13秒前
wanci应助wangdafa采纳,获得10
13秒前
竹子co完成签到,获得积分10
13秒前
steventj完成签到,获得积分10
13秒前
yz完成签到 ,获得积分10
14秒前
朴实山兰完成签到,获得积分10
15秒前
tkkdy发布了新的文献求助10
15秒前
蓁蓁发布了新的文献求助10
15秒前
醉熏的鑫发布了新的文献求助10
16秒前
独家双层汉堡完成签到,获得积分10
16秒前
Li完成签到,获得积分10
17秒前
xcx发布了新的文献求助10
17秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds第二卷 1200
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038657
求助须知:如何正确求助?哪些是违规求助? 3576306
关于积分的说明 11375198
捐赠科研通 3306108
什么是DOI,文献DOI怎么找? 1819379
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066