Ratiometric fluorescence sensor for organophosphorus pesticide detection based on opposite responses of two fluorescence reagents to MnO2 nanosheets

荧光 化学 检出限 试剂 氯化丹酯 乙酰胆碱酯酶 线性范围 分解 光化学 色谱法 质谱法 有机化学 物理 量子力学 衍生化
作者
Tiantian Yao,Anran Liu,Yong Liu,Min Wei,Wei Wei,Songqin Liu
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:145: 111705-111705 被引量:98
标识
DOI:10.1016/j.bios.2019.111705
摘要

The detection of organophosphorus pesticides (OPs) has received considerable attention for their great harm to human beings. Herein, a novel ratiometric fluorescence biosensor was constructed for the determination of OPs by using Scopoletin (SC) and Amplex Red (AR) as probe pairs that have opposite responses to MnO2 nanosheets (MnO2 NS). MnO2 NS possess peroxidase-like catalytic activity, which could quench the fluorescence of SC as well as enhance the fluorescence of the non-fluorescent substance AR by oxidation. In the absence of OPs, acetylcholinesterase (AChE) hydrolyzed acetylcholine chloride (ATCh) into choline (TCh) and acetate. TCh led the decomposition of MnO2 NS to manganese ions (Mn2+), increasing signal of SC and decreasing signal of AR. In the presence of OPs, the activity of AChE was inhibited and the decomposition of MnO2 NS was hindered, therefore the fluorescence intensity of SC was weak and the fluorescence intensity of AR had an obvious increase. Moreover, under the optimal conditions, the ratio of fluorescence intensity response recorded on the AR/SC increases with increasing the concentration of DDVP. The method has wider linear range of 5.0 pg/mL ∼500 ng/mL with a detection limit of 1.6 pg/mL, which is superior to previously reported methods. This strategy has also been applied to a visual observation based on the color change of the solution under UV light.
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