Relationship between Alzheimer's disease and mitochondria coenzyme II Gene.

We aimed to investigate the relevance between Alzheimer's disease (AD) and gene mutations of mitochondrial cytochrome oxidase subunit III (COX3) and coenzyme II (ND2), and to provide genetic markers for the diagnosis of Alzheimer's disease (AD) and further provide some feasible basis for preventive treatment. Polymerase chain reaction-restriction fragment length polymorphism technique was used, and genotypes and gene frequencies were detected in 60 patients with Alzheimer's disease (AD), who meet the ICD-10 diagnostic criteria (AD group), 10 AD families and 60 normal old people (control group). (1) Gene variation on nt5460 gene locus of mitochondria ND2 of the patient group is G→A, and the variation rate is 13.3%, P=0.006 < 0.05. Gene variation G→A of the patient group perfomred statistical significance. (2) In the families, it is also found that in the gene variation of G→A, the variation rate is 33.3%, P > 0.05. There is obvious gene variation in the families, but this variation does not perform statistical significance. (3) There is no gene variation on nt9861 gene locus of mitochondria COX3 of the patient group. Gene variation of T→C is not found both in the patient group and the control group. There is possible a gene mutation of G→A on nt5460 gene locus of mitochondria ND2 of the AD patients. Although gene mutation of G→A is found in the families, it performed no statistical significance. At the same time, it is found that there is no relation between AD patients and Gene variation of T→C on nt9861 gene locus of mitochondria COX3.