Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser Baeri and A. ruthenus

Journal of Applied IchthyologyVolume 12, Issue 2 p. 107-112 Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser Baeri and A. ruthenus L. I. Tsvetkova, L. I. Tsvetkova Laboratory of Cryobiology, All-Russian Institute of Pond Fisheries, Rybnoye, Dmitrov Region, Moscow Area, RussiaSearch for more papers by this authorJ. Cosson, J. Cosson CNRS URA 671, Station Marine, Villefranche sur Mer, FranceSearch for more papers by this authorO. Linhart, Corresponding Author O. Linhart Research Institute of Culture and Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, Vodnany, Czech RepublicAuthors' address: O. Linhart, Research Institute of Fish Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, 38925 Vodnany, Czech RepublicSearch for more papers by this authorR. Billard, R. Billard Laboratoire d'Ichtyologie, Museum National d'Histoire Naturelle, Paris Cedex, FranceSearch for more papers by this author L. I. Tsvetkova, L. I. Tsvetkova Laboratory of Cryobiology, All-Russian Institute of Pond Fisheries, Rybnoye, Dmitrov Region, Moscow Area, RussiaSearch for more papers by this authorJ. Cosson, J. Cosson CNRS URA 671, Station Marine, Villefranche sur Mer, FranceSearch for more papers by this authorO. Linhart, Corresponding Author O. Linhart Research Institute of Culture and Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, Vodnany, Czech RepublicAuthors' address: O. Linhart, Research Institute of Fish Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, 38925 Vodnany, Czech RepublicSearch for more papers by this authorR. Billard, R. Billard Laboratoire d'Ichtyologie, Museum National d'Histoire Naturelle, Paris Cedex, FranceSearch for more papers by this author First published: July 1996 https://doi.org/10.1111/j.1439-0426.1996.tb00071.xCitations: 77AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The Siberian sturgeon (Acipenser baeri) and the sterlet (A. ruthenus) were injected with dried sturgeon pituitary (2 mg kg−1), yielding 24 h later respectively 1.71 ± 0.5 and 1.65 ± 0.5 1011 spermatozoa kg−1 body weight. Spermatozoa were best activated with a solution of Tris HC1 50 mM, pH 8.0. The percentage of activated cells was 88 ± 4.4 in A. baeri (n = 5) and 68 ± 19 in A. ruthenus. (n = 5). In A. baeri, immediately after activation, the beat frequency of the flagellum and the mean velocity were in the range of 48–52 Hz and 100–300 μm−1s, respectively. The beat frequency declined to 15 Hz at 30–40 and velocity to 100 μm s−1 at 60 s post-activation. Only a small percentage of the spermatozoa remained motile after 3–4 min. In all cases spermatozoa showed mostly quasi-linear trajectories. Sperm was frozen in liquid nitrogen vapor immediately after dilution 1 v: 1 v in a cryopreservation medium (23.4 mM saccharose, 118 mM Tris-HCl pH 8.0, 20% egg yolk to which 15% DMSO were added). After fast-thawing procedure (25 s at 40°C), the percentage of motile spermatozoa (once activated in 118 mM Tris-HCl, pH 8.0) decreased to 23 ± 8.8 in A. baeri and to 15 ± 11 in A. ruthenus. The fertilizing capacity also decreased: 53 ± 8.3% vs. 89 ± 7.6% in control (P < 0.05) in A. baeri and 23 ± 11% vs 53 ± 8.3% in control (P < 0.05) in A. ruthenus. The motility pattern of the surviving frozen/thawed spermatozoa was the same as in fresh spermatozoa. References Billard, R.; Cosson, M. P., 1990: The energetics of fish sperm motility. Controls of Sperm Motility: Biological and Clinical Aspects, CRC Press, Boston : 153–171. Billard, R.; Cosson, J.; Crim, L. W., 1993: Motility of fresh and aged halibut sperm. Aquat. Living Ressour. 61, 67–75. Billard, R.; Cosson, J.; Perchec, G.; Linhart, O., 1995: Biology of sperm and artificial reproduction in carp. Aquaculture 129, 95–112. Brown, G. G.; Mims, S. D., 1995: Storage, transportation, and fertility of undiluted and diluted paddlefish milt. Prog. Fish-Cult 57, 64–69. Burcev, I. A.; Cerebrjackova, A. V., 1969: First results of cryopreservation in sturgeon spermatozoa. In: Trudy molodych utchenych vesesojuzn. 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