Aqueous Two Phase Systems for the Recovery of Biomolecules – A Review
生物分子
水溶液
萃取(化学)
化学
色谱法
核酸
双水相体系
相(物质)
生物化学
有机化学
作者
S. Thirupathi Kumara Raja,V. Ramachandra Murty,Thivaharan Varadavenkatesan,Vinayagam Rajasekar,Ramesh Vinayagam
出处
期刊:Science and technology日期:2012-08-31卷期号:1 (1): 7-16被引量:233
标识
DOI:10.5923/j.scit.20110101.02
摘要
Aqueous two phase system (ATPS) is a liquid â liquid extraction method which employs two aqueous phases having applications in the field of biotechnology for the separation and purification of biological materials such as proteins, enzymes, nucleic acids, virus, antibodies and cell organelles. This review discusses the basic principles of ATPS, the factors affecting partitioning, optimization by design of experiments and the recent applications like extractive fermentation, membrane supported liquid â liquid extraction and aqueous two phase floatation. A comparative study between ATPS and other conventional methods is also discussed. The emphasis is given to PEG/salt two-phase systems because of the low cost of the system.