脊髓
轴突
再生(生物学)
脊髓损伤
神经营养因子
解剖
中枢神经系统
神经科学
生物
细胞生物学
生物化学
受体
作者
Mingyong Gao,Paul Lu,Bridget Bednark,Dan Lynam,J. M. Conner,Jeff Sakamoto,Mark H. Tuszynski
出处
期刊:Biomaterials
[Elsevier]
日期:2013-02-01
卷期号:34 (5): 1529-1536
被引量:136
标识
DOI:10.1016/j.biomaterials.2012.10.070
摘要
Bioengineered scaffolds have the potential to support and guide injured axons after spinal cord injury, contributing to neural repair. In previous studies we have reported that templated agarose scaffolds can be fabricated into precise linear arrays and implanted into the partially injured spinal cord, organizing growth and enhancing the distance over which local spinal cord axons and ascending sensory axons extend into a lesion site. However, most human injuries are severe, sparing only thin rims of spinal cord tissue in the margins of a lesion site. Accordingly, in the present study we examined whether template agarose scaffolds seeded with bone marrow stromal cells secreting Brain-Derived Neurotrophic Factor (BDNF) would support regeneration into severe, complete spinal cord transection sites. Moreover, we tested responses of motor axon populations originating from the brainstem. We find that templated agarose scaffolds support motor axon regeneration into a severe spinal cord injury model and organize axons into fascicles of highly linear configuration. BDNF significantly enhances axonal growth. Collectively, these findings support the feasibility of scaffold implantation for enhancing central regeneration after even severe central nervous system injury.
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