肾单位
生物
祖细胞
间充质
人口
祖细胞
多能干细胞
细胞生物学
肾脏发育
入侵
肾干细胞
肾
干细胞
间充质干细胞
内分泌学
遗传学
胚胎干细胞
胚胎发生
医学
原肠化
胚胎
基因
环境卫生
作者
Akio Kobayashi,M. Todd Valerius,Joshua W. Mugford,Thomas J. Carroll,Michelle Self,Guillermo Oliver,Andrew P. McMahon
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2008-08-01
卷期号:3 (2): 169-181
被引量:833
标识
DOI:10.1016/j.stem.2008.05.020
摘要
Nephrons, the basic functional units of the kidney, are generated repetitively during kidney organogenesis from a mesenchymal progenitor population. Which cells within this pool give rise to nephrons and how multiple nephron lineages form during this protracted developmental process are unclear. We demonstrate that the Six2-expressing cap mesenchyme represents a multipotent nephron progenitor population. Six2-expressing cells give rise to all cell types of the main body of the nephron during all stages of nephrogenesis. Pulse labeling of Six2-expressing nephron progenitors at the onset of kidney development suggests that the Six2-expressing population is maintained by self-renewal. Clonal analysis indicates that at least some Six2-expressing cells are multipotent, contributing to multiple domains of the nephron. Furthermore, Six2 functions cell autonomously to maintain a progenitor cell status, as cap mesenchyme cells lacking Six2 activity contribute to ectopic nephron tubules, a mechanism dependent on a Wnt9b inductive signal. Taken together, our observations suggest that Six2 activity cell-autonomously regulates a multipotent nephron progenitor population.
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