Quantification of subchondral bone changes in a murine osteoarthritis model using micro-CT.

骨关节炎 软骨下骨 X射线显微断层摄影术 医学 胫骨 软骨 胶原酶 骨骺 解剖 舱室(船) 病理 关节软骨 放射科 化学 替代医学 生物化学 地质学 海洋学
作者
Sander M. Botter,Gerjo J. V. M. van Osch,J.H. Waarsing,Judd S. Day,Jan A.N. Verhaar,Huibert A. P. Pols,Johannes P.T.M. van Leeuwen,H. Weinans
出处
期刊:PubMed 卷期号:43 (3,4): 379-88 被引量:99
链接
标识
摘要

In the past few years there has been a considerable interest in the role of bone in osteoarthritis. Despite the increasing evidence of the involvement of bone in osteoarthritis, it remains very difficult to attribute the cause or effect of changes in subchondral bone to the process of osteoarthritis. Although osteoarthritis in mice provides a useful model to study changes in the subchondral bone, detailed quantification of these changes is lacking. Therefore, the goal of this study was to quantify subchondral bone changes in a murine osteoarthritis model by use of micro-computed tomography (micro-CT). We induced osteoarthritis-like characteristics in the knee joints of mice using collagenase injections, and after four weeks we calculated various 3D morphometric parameters in the epiphysis of the proximal tibia. The collagenase injections caused cartilage damage, visible in histological sections, particularly on the medial tibial plateau. Micro-CT analysis revealed that the thickness of the subchondral bone plate was decreased both at the lateral and the medial side. The trabecular compartment demonstrated a small but significant reduction in bone volume fraction compared to the contralateral control joints. Trabeculae in the collagenase-injected joints were thinner but their shape remained rod-like. Furthermore, the connectivity between trabeculae was reduced and the trabecular spacing was increased. In conclusion, four weeks after induction of osteoarthritis in the murine knee subtle but significant changes in subchondral bone architecture could be detected and quantified in 3D with micro-CT analysis.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
maizhan完成签到,获得积分10
1秒前
默认用户名完成签到,获得积分10
2秒前
3秒前
leei完成签到,获得积分10
4秒前
meng123发布了新的文献求助10
4秒前
小马甲应助c123采纳,获得10
5秒前
莎莎士比亚完成签到,获得积分10
7秒前
LOST完成签到 ,获得积分10
7秒前
8秒前
袁凯文发布了新的文献求助10
8秒前
共享精神应助老菜鸟321采纳,获得10
8秒前
WUWEI发布了新的文献求助10
9秒前
xiaoW完成签到,获得积分10
9秒前
11秒前
牛太虚完成签到,获得积分10
11秒前
SciGPT应助科多兽骑士采纳,获得10
11秒前
13秒前
gjm完成签到,获得积分10
13秒前
SciGPT应助zj采纳,获得10
14秒前
Ava应助阿湫采纳,获得10
14秒前
meng123完成签到,获得积分20
15秒前
x5kyi完成签到,获得积分10
16秒前
爆米花应助肖遥采纳,获得10
17秒前
Xx完成签到,获得积分10
17秒前
17秒前
20秒前
烟里戏完成签到 ,获得积分10
22秒前
shuangfeng1853完成签到 ,获得积分10
22秒前
林子青发布了新的文献求助10
22秒前
23秒前
aa完成签到,获得积分10
23秒前
CXC完成签到,获得积分10
23秒前
25秒前
Zzz发布了新的文献求助10
25秒前
上官若男应助袁凯文采纳,获得10
26秒前
26秒前
褚晣完成签到,获得积分10
26秒前
ATTENTION完成签到,获得积分10
27秒前
27秒前
周欣玙完成签到,获得积分10
27秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038524
求助须知:如何正确求助?哪些是违规求助? 3576221
关于积分的说明 11374737
捐赠科研通 3305912
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892688
科研通“疑难数据库(出版商)”最低求助积分说明 815048