间充质干细胞
血管内皮生长因子
骨形态发生蛋白2
骨形态发生蛋白
基因传递
干细胞
血管内皮生长因子A
医学
生长因子
细胞生物学
遗传增强
癌症研究
分子生物学
生物
血管内皮生长因子受体
体外
内科学
基因
遗传学
受体
作者
Jesse Seamon,Xiuli Wang,Fuai Cui,Tom Keller,Abhijit S. Dighe,Gary Balian,Quanjun Cui
出处
期刊:Bone Marrow Research
[Hindawi Limited]
日期:2013-02-26
卷期号:2013: 1-9
被引量:17
摘要
The combined delivery of mesenchymal stem cells (MSCs), vascular endothelial growth factor (VEGF), and bone morphogenetic protein (BMP) to sites of bone injury results in enhanced repair compared to the administration of a single factor or a combination of two factors. Based on these findings, we hypothesized that coexpression of VEGF and BMP-6 genes would enhance the osteoblastic differentiation of rat bone-marrow-derived stem cells (rMSCs) and osteogenesis by comparison to rMSCs that do not express VEGF and BMP-6. We prepared a GFP tagged adenovirus vector (Ad-VEGF+BMP-6) that contained DNA encoding the hVEGF and hBMP-6 genes. rMSCs were transduced with the virus, and the successful transduction was confirmed by green fluorescence and by production of VEGF and BMP-6 proteins. The cells were cultured to assess osteoblastic differentiation or administered in the Fischer 344 rats to assess bone formation. Mineralization of rMSCs transduced with Ad-VEGF+BMP-6 was significantly enhanced over the nontransduced rMSCs. Only transduced rMSCs could induce osteogenesis in vivo , whereas Ad-VEGF+BMP-6 or nontransduced rMSCs alone did not induce osteogenesis. The data suggests that the combined delivery of MSCs, VEGF, and BMP-6 is an attractive option for bone repair therapy.
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