Cultured cell-derived extracellular matrix scaffolds for tissue engineering

组织工程 细胞外基质 间充质干细胞 软骨细胞 脚手架 细胞生物学 软骨发生 成纤维细胞 生物医学工程 软骨 材料科学 细胞粘附 细胞培养 粘附 化学 体外 生物 解剖 医学 生物化学 复合材料 遗传学
作者
Hongxu Lu,Takashi Hoshiba,Naoki Kawazoe,Ichie Koda,Minghui Song,Guoping Chen
出处
期刊:Biomaterials [Elsevier]
卷期号:32 (36): 9658-9666 被引量:211
标识
DOI:10.1016/j.biomaterials.2011.08.091
摘要

Cell-derived extracellular matrix (ECM) scaffolds have received considerable interest for tissue engineering applications. In this study, ECM scaffolds derived from mesenchymal stem cell (MSC), chondrocyte, and fibroblast were prepared by culturing cells in a selectively removable poly(lactic-co-glycolic acid) (PLGA) template. These three types of ECM scaffolds were used for in vitro cultures of MSC and fibroblasts to examine their potential as scaffolds for cartilage and skin tissue engineering. The MSC were cultured in MSC- and chondrocyte-derived ECM scaffolds. The ECM scaffolds supported cell adhesion, promoted both cell proliferation and the production of ECM and demonstrated a stronger stimulatory effect on the chondrogenesis of MSC compared with a conventional pellet culture method. Histological and immunohistochemical staining indicated that cartilage-like tissues were regenerated after the MSC were cultured in ECM scaffolds. Fibroblasts were cultured in the fibroblast-derived ECM scaffolds. Fibroblasts proliferated and produced ECM to fill the pores and spaces in the scaffold. After 2 weeks of culture, a uniform multilayered tissue was generated with homogenously distributed fibroblasts. Cell-derived ECM scaffolds have been demonstrated to facilitate tissue regeneration and will be a useful tool for tissue engineering.
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