The Effect of the CAM and CAM-Dependent Kinase on the Expression of c-fos Gene in the Spinal Cord Matter

Objective To study the effect of CAM and CAM-dependent kinase on the the expression of c-fos gene in the spinal cord matter.Methods After the cultured spinal cord slices in high calcium ions situation treated with CAM and CAM-dependent kinase at the time from 30 minuts to 12 hour,the hybrid in situ was studied to reveal the expression of c-fos gene.Results The strongest expression was found in the high calcium ions group,and the expression reached the maxium at 4～6 h after treatment.The enhanced expression happened in the TFP group and the KN-62 group at the 6h after treatment.Conclusions The action of calcium ions inducing the expression of c-fos may be realized by compounding with CAM,then rousing CAM-PK.