Curcumin-encapsulating Nanogels as an Effective Anticancer Formulation for Intracellular Uptake.

姜黄素 纳米凝胶 纳米载体 细胞毒性 化学 吖啶橙 药物输送 泊洛沙姆 生物利用度 癌细胞 药理学 阿霉素 生物化学 细胞凋亡 癌症 体外 医学 有机化学 外科 内科学 聚合物 化疗 共聚物
作者
Anna E. Reeves,Serguei V. Vinogradov,Phil Morrissey,Mitchell Chernin Chernin,Mansoor M. Ahmed
出处
期刊:PubMed 卷期号:7 (3): 25-40 被引量:39
标识
DOI:10.4255/mcpharmacol.15.04
摘要

Nanoscale drug delivery systems represent an attractive strategy to improve both the efficacy and safety of anticancer drugs. In this work, we describe nanoformulation of curcumin, a most potent natural anticancer compound capable of killing cancer cells while sparing the normal tissues. Since curcumin is a natural hydrophobic polyphenol, it has a low aqueous solubility and bioavailability, which are challenging to its therapeutic efficacy. We developed and evaluated a novel colloidal nanogel carrier for encapsulation of curcumin to increase its solubility and cytotoxicity. Amphiphilic Poloxamer-cationic network in the nanogel NG127 was designed to efficiently encapsulate curcumin. Homogenous drug complexes were obtained with 20-25% content of curcumin and the particle size of ca. 150 nm. Using ImageStream multispectral imaging flow cytometry, we demonstrated that the curcumin-nanogel formulation (C-NG) was readily internalized into MDA-231 breast cancer cells. A real-time cell growth electronic sensing assay was used to measure proliferation responses of various breast cancer cells to C-NG treatments. Our results indicated that the C-NG formulation was 70-85% more effective in inhibiting growth, at concentrations lower than IC50 of free curcumin. This was also confirmed morphologically by modified acridine orange/ethidium bromide staining and fluorescent microscopy. Importantly, nanocarrier NG127 alone displayed practically no cytotoxicity. We conclude that nanogel carriers offer an innovative way to encapsulate curcumin and to obtain more effective anticancer therapeutics than curcumin alone with a potential to specific tumor targeting, such as using antibodies against surface receptors specific to breast cancer cells.
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