Influence of temperature, pH and organics on ANAMMOX activity was investigated through measuring the anaerobic ammonia oxidation rate of sludge. Results show that temperature and pH could influence ANAMMOX activity dramatically. The optimal temperature was between 30-35 degrees C , and the relationship between the anaerobic ammonia oxidation rate and temperature can be described by revised Arrhenius formula during 20-30 degreee C. The optimal pH was between 7.5 - 8.3, and the relationship between the anaerobic ammonia oxidation rate and pH can be described by two substrates and two inhibitors model during pH 7.0 - 9.0. There was substrata competition between the heterotrophic denitrification bacteria and ANAMMOX bacteria when organics in the