Objective To study the chemical constituents in the roots of Goniothalamus cheliensis.Methods Silica gel,ODS,and Sephadex LH-20 column chromatographic techniques were used to isolate and purify the chemical constituents and their structures were elucidated by spectral analyses.Results Seven compounds were isolated and identified as acetylgoniofupyrone A(1),protocatechuic acid(2),pinocembrin(3),pinoresinol(4),goniodiol(5),8-epi-goniotriol(6),and cardiobutanolide(7).Conclusion Compound 1 is a new goniofupyrone-type of styryllactone,named acetylgoniofupyrone A.