Cholesterol biosensor based on a plastic optical fibre with sol–gel: structural analysis and sensing properties

In this paper, we report the design and characterization of an optical fibre cholesterol biosensor by using sol–gel immobilization technique. The cholesterol enzyme is encapsulated inside of the sol–gel film onto an end of a plastic optical fibre. Two film deposition methods (Dip-Coating and Immersion) were studied. The morphology analysis and sensing properties permit us to determine the best film deposition to sense cholesterol concentration. The range of measured is 4.4–5.2 mM in real time and our results were validated by comparing them with other previously published results. The biosensor is portable, simple cheap, and easy to use.