生物合成
聚酮
基因簇
立体化学
GenBank公司
基因
登录号(图书馆学)
羟基化
拉伤
聚酮合酶
突变体
化学
链霉菌
生物化学
生物
遗传学
酶
细菌
解剖
作者
Jianxiong Wang,Xiaoman Li,Chunhua Lu,Yuemao Shen
出处
期刊:ChemBioChem
[Wiley]
日期:2017-11-29
卷期号:19 (3): 256-262
被引量:5
标识
DOI:10.1002/cbic.201700528
摘要
Abstract The ast gene cluster (GenBank accession numbers KF813023.1 and KP284551) was characterized to be responsible for the biosynthesis of ansatrienins in Streptomyces sp. XZQH13, which contains astC , astF1 , and astF2 genes involved in the assembly of the N ‐cyclohexanoyl d ‐alanyl side chain and the hydroxylation of C‐19, respectively. Further to investigating the biosynthetic mechanism of ansatrienins, herein we constructed the mutant strains XZQH13OEΔ astF2 and XZQH13OEΔ astC Δ astF2 . Three new ansatrienin analogues, namely, ansatrienols I–K ( 1 – 3 ), along with trienomycinol ( 4 ) and 3‐ O ‐demethyltrienomycinol ( 5 ), were isolated from the XZQH13OEΔ astC Δ astF2 strain, and trienomycin A ( 6 ) and trienomycin G ( 7 ) were isolated from the XZQH13OEΔ astF2 strain. Their structures were determined by a combination of high‐resolution MS (ESI) and 1D and 2D NMR spectroscopy. Accordingly, a pathway for the biosynthesis of these new ansatrienins was proposed.
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