Spatial interaction of tumor cells and regulatory T cells correlates with survival in non-small cell lung cancer

CD8型 肿瘤微环境 医学 肺癌 FOXP3型 川地68 多路复用 免疫系统 病理 单变量分析 组织微阵列 免疫组织化学 癌症研究 内科学 生物 免疫学 多元分析 生物信息学
作者
Souptik Barua,Penny Fang,Amrish Sharma,Junya Fujimoto,Ignacio I. Wistuba,Arvind Rao,Steven H. Lin
出处
期刊:Lung Cancer [Elsevier BV]
卷期号:117: 73-79 被引量:155
标识
DOI:10.1016/j.lungcan.2018.01.022
摘要

Objectives To determine the prognostic significance of spatial proximity of lung cancer cells and specific immune cells in the tumor microenvironment. Materials and methods We probed formalin-fixed, paraffin-embedded (FFPE) tissue microarrays using a novel tyramide signal amplification multiplexing technique labelling CD8, CD4, Foxp3, and CD68+ cells. Each multiplex stained immunohistochemistry slide was digitally processed by Vectra INFORMS software, and an X- and Y-coordinate assigned to each labeled cell type. The abundance and spatial location of each cell type and their proximity to one another was analyzed using a novel application of the G-cross spatial distance distribution method which computes the probability of finding at least one immune cell of any given type within a rμm radius of a tumor cell. Cox proportional hazards multiple regression was used for multivariate analysis of the influence of proximity of lymphocyte types. Results Pathologic tumor specimens from 120 NSCLC patients with pathologic tumor stage I–III disease were analyzed. On univariate analysis, age (P = .0007) and number of positive nodes (P = .0014) were associated with overall survival. Greater area under the curve (AUC) of the G-cross function for tumor cell-Treg interactions was significantly associated with worse survival adjusting for age and number of positive nodes (HR 1.52 (1.11–2.07), P = .009). Greater G-cross AUC for T-reg-CD8 was significantly associated with better survival adjusting for age and number of positive lymph nodes (HR 0.96 (0.92–0.99), P = .042). Conclusion Increased infiltration of regulatory T cells into core tumor regions is an independent predictor of worse overall survival in NSCLC. However, increased infiltration of CD8+ cytotoxic T cells among regulatory T cells seems to mitigate this effect and was significantly associated with better survival. Validation of the G-cross method of measuring spatial proximity between tumor and immune cell types and exploration of its use as a prognostic factor in lung cancer treatment is warranted.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Xiaoxiao发布了新的文献求助20
1秒前
醉熏的鑫发布了新的文献求助10
2秒前
迅速海云完成签到,获得积分10
2秒前
2秒前
栗子的小母牛完成签到,获得积分10
3秒前
dh完成签到,获得积分10
6秒前
岩墩墩完成签到,获得积分10
7秒前
克姑美完成签到 ,获得积分10
10秒前
pangao完成签到,获得积分10
10秒前
ysssbq完成签到,获得积分10
12秒前
13秒前
上好佳完成签到 ,获得积分10
13秒前
大模型应助Yeong采纳,获得10
14秒前
量子星尘发布了新的文献求助30
14秒前
15秒前
123完成签到,获得积分10
15秒前
谢陈完成签到 ,获得积分10
16秒前
lilili完成签到,获得积分10
17秒前
18秒前
xiaoying发布了新的文献求助10
18秒前
SciGPT应助Eric_Liuzy采纳,获得10
19秒前
liu完成签到 ,获得积分10
19秒前
qixiaoqi发布了新的文献求助10
20秒前
21秒前
A溶大美噶发布了新的文献求助10
21秒前
22秒前
22秒前
23秒前
24秒前
25秒前
kevin发布了新的文献求助10
25秒前
满意的初南完成签到 ,获得积分10
26秒前
橙橙橙发布了新的文献求助10
26秒前
万能图书馆应助EVEN采纳,获得10
26秒前
26秒前
Yeong发布了新的文献求助10
27秒前
范先生发布了新的文献求助10
28秒前
disciple完成签到,获得积分10
29秒前
沉默凌寒完成签到,获得积分10
29秒前
cc完成签到,获得积分10
29秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038569
求助须知:如何正确求助?哪些是违规求助? 3576279
关于积分的说明 11374944
捐赠科研通 3305979
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815048