Exogenous fragmented DNA acts as a damage-associated molecular pattern (DAMP) inducing changes in CpG DNA methylation and defence-related responses in Lactuca sativa

生物 潮湿 DNA甲基化 拉图卡 表观遗传学 DNA损伤 CpG站点 DNA 遗传学 植物 基因 基因表达 物理 气象学
作者
Isaac Vega-Muñoz,Ana A. Feregrino‐Pérez,Irineo Torres‐Pacheco,Ramón Gerardo Guevara-González
出处
期刊:Functional Plant Biology 卷期号:45 (10): 1065-1065 被引量:38
标识
DOI:10.1071/fp18011
摘要

Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self-nonself recognition in response to attack by biotic and abiotic stresses. Extracellular DNA has emerged as a possible DAMP. As a DAMP DNA seems to function depending on the phylogenetic scale and has been tested in a few plant species. This study aimed to evaluate the possible role of self DNA (sDNA) as a DAMP by analysing changes in CpG DNA methylation and defence-related responses in lettuce (Lactuca sativa L.) as a model plant. sDNA and nonself DNA (nsDNA) from Capsicum chinense Murray (both species belong to the same clade, Asterids) stimulated aberrant seed germination and root growth in lettuce seedlings. Similar resultswere obtained with nsDNA obtained from Acaciella angustissima (Mill.) Britton & Rose plants (belonging to the clade Rosids I), although at significantly higher concentrations. Moreover, in most cases, this behaviour was correlated with hypomethylation of CpG DNA as well as defence responses measured as altered gene expression associated with oxidative burst and production of secondary metabolites (phenylpropanoids) related to coping with stress conditions. Our results suggested that extracellular and fragmented DNA has a role as a DAMP depending on phylogenetic closeness in plants as lettuce, inducing epigenetic, genetic and biochemical changes within the plant. The importance of our results is that, for the first time, they demonstrate that sDNA acts as a DAMP in plants, changing CpG DNA methylation levels as well as increasing the production of secondary metabolites associated with defence responses to stress.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
热心凡雁发布了新的文献求助20
2秒前
2秒前
我是老大应助听弦采纳,获得10
2秒前
as发布了新的文献求助10
2秒前
2秒前
荼蘼如雪完成签到,获得积分10
3秒前
火星上易梦完成签到,获得积分20
3秒前
5秒前
5秒前
许红发布了新的文献求助10
5秒前
6秒前
包子妹妹发布了新的文献求助10
7秒前
7秒前
7秒前
雪白飞丹发布了新的文献求助10
8秒前
请叫我表情帝完成签到,获得积分10
8秒前
飘逸果汁完成签到 ,获得积分10
10秒前
Jasper应助李新祎采纳,获得10
10秒前
恋风恋歌发布了新的文献求助30
10秒前
伏西楠行发布了新的文献求助10
10秒前
11秒前
杨紫琴发布了新的文献求助10
12秒前
ding应助包子妹妹采纳,获得10
13秒前
13秒前
顾矜应助杨青柳采纳,获得10
13秒前
mellow完成签到,获得积分10
14秒前
15秒前
15秒前
eleven完成签到,获得积分20
15秒前
15秒前
15秒前
Lucas应助LuoYR@SZU采纳,获得10
16秒前
前寒武完成签到,获得积分10
16秒前
17秒前
17秒前
咕噜完成签到,获得积分10
17秒前
小二郎应助跳跃富采纳,获得10
17秒前
gnufgg发布了新的文献求助200
17秒前
小阿博发布了新的文献求助10
17秒前
高分求助中
Impact of Mitophagy-Related Genes on the Diagnosis and Development of Esophageal Squamous Cell Carcinoma via Single-Cell RNA-seq Analysis and Machine Learning Algorithms 2000
Evolution 1100
How to Create Beauty: De Lairesse on the Theory and Practice of Making Art 1000
Gerard de Lairesse : an artist between stage and studio 670
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 550
T/CAB 0344-2024 重组人源化胶原蛋白内毒素去除方法 500
[Procedures for improving absorption properties of polystyrene microtest plates by coating with nitrocellulose] 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 2983747
求助须知:如何正确求助?哪些是违规求助? 2644811
关于积分的说明 7139961
捐赠科研通 2278107
什么是DOI,文献DOI怎么找? 1208566
版权声明 592176
科研通“疑难数据库(出版商)”最低求助积分说明 590449