A novel aptamer-based online magnetic solid phase extraction method for simultaneous determination of urinary 8-hydroxy-2′-deoxyguanosine and monohydroxylated polycyclic aromatic hydrocarbons

化学 吸附剂 萃取(化学) 检出限 吸附 色谱法 固相萃取 适体 脱氧鸟苷 磁性纳米粒子 高效液相色谱法 纳米颗粒 纳米技术 加合物 生物 有机化学 材料科学 遗传学
作者
Haijiao Gan,Hui Xu
出处
期刊:Talanta [Elsevier BV]
卷期号:201: 271-279 被引量:24
标识
DOI:10.1016/j.talanta.2019.04.004
摘要

In this work, an innovative aptamer-based magnetic adsorbent (Fe3O4@PDA-aptamer MNPs) was prepared by hydrothermal synthesis method followed by the surface functionalization of nanoparticles. After fixing in a steel stainless tube as sorbent of magnetic solid phase extraction (MSPE), an online magnetic solid phase extraction-high performance liquid chromatography-mass spectrometry (online-MSPE-HPLC-MS) method was developed and applied for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) simultaneously in urine. The synthesized sorbent presented outstanding features, including large specific surface area, high enrichment capacity and excellent stability. High throughput analysis can be achieved by affinity-specific adsorption of 8-OHdG and non-specific adsorption of OH-PAHs at the same time. In addition, online MSPE can greatly simplify the analysis process, reduce human errors and enhance the sensitivity. When compared with offline MSPE, a sensitivity enhancement of 30–400 times was obtained for the online method. Some experimental parameters such as the amount of the sorbent, sampling flow rate and sample volume, were optimized systematically. Under the optimal conditions, the limits of detection (LOD) were in the range of 0.028–0.114 ng mL−1, and the correlation coefficients (R2) were higher than 0.9962. The relative standard deviations (RSDs) were less than 16.1% (n = 5) and the recoveries ranged from 71% to 116%. The above results show that the rapid, sensitive and automated online-MSPE-HPLC-MS method has potential application in the simultaneous determination of 8-OHdG and PAHs in complex sample matrix to assess the environmental exposure level.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
头号玩家发布了新的文献求助10
1秒前
大个应助xubee采纳,获得10
2秒前
3秒前
SQDHZJ完成签到,获得积分10
3秒前
yokliang发布了新的文献求助10
5秒前
轻松的惜芹应助聪明面包采纳,获得200
7秒前
7秒前
fanmo完成签到 ,获得积分0
8秒前
陈美净发布了新的文献求助10
8秒前
jh完成签到,获得积分10
9秒前
August完成签到,获得积分10
9秒前
12秒前
小张发布了新的文献求助10
12秒前
12秒前
LY发布了新的文献求助10
13秒前
个性的冬卉完成签到,获得积分10
13秒前
传奇3应助Gaara0504采纳,获得10
14秒前
NexusExplorer应助科研通管家采纳,获得10
14秒前
传奇3应助科研通管家采纳,获得10
14秒前
reirei应助科研通管家采纳,获得10
14秒前
Hello应助科研通管家采纳,获得10
14秒前
Lucas应助科研通管家采纳,获得50
14秒前
14秒前
英姑应助科研通管家采纳,获得10
15秒前
Hello应助科研通管家采纳,获得10
15秒前
Owen应助科研通管家采纳,获得10
15秒前
情怀应助科研通管家采纳,获得10
15秒前
丘比特应助科研通管家采纳,获得10
15秒前
我是老大应助科研通管家采纳,获得10
15秒前
上官若男应助科研通管家采纳,获得10
15秒前
15秒前
丘比特应助科研通管家采纳,获得10
15秒前
15秒前
15秒前
GXX应助科研通管家采纳,获得10
15秒前
Newt应助科研通管家采纳,获得20
15秒前
Mea完成签到,获得积分10
17秒前
yuaasusanaann发布了新的文献求助10
18秒前
nura发布了新的文献求助10
18秒前
小6s发布了新的文献求助10
18秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976210
求助须知:如何正确求助?哪些是违规求助? 3520366
关于积分的说明 11203088
捐赠科研通 3256965
什么是DOI,文献DOI怎么找? 1798570
邀请新用户注册赠送积分活动 877738
科研通“疑难数据库(出版商)”最低求助积分说明 806516