A Validated RP-HPLC Method for Determination of Rose Bengal in Bulk and Surgical Strips

A simple validated RP-HPLC method for determination of Rose Bengal (RB) Surgical strips which are used for staining of damaged cells of eye. The successful separation of RB was achieved on a Cromosil 100-5μm column (4.6 x 250mm with 5μm diameter) and detector of UV 262nm 1.0ml/min as a flow rate, and 20μl as an injector volume. The mobile phase of proposed method was methanol and phosphate Buffer of pH 8.0 in 50:50 (v/v) ratios. The linearity of method was determined in the range of 8-40ppm. The regression coefficient; LOD and LOQ were respectively 0.9987, 1ppm and 3ppm. It was validated according to the guidelines of ICH and USP.