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Isolation, characterization, and application of a novel specific Salmonella bacteriophage in different food matrices

沙门氏菌 长尾病毒科 噬菌体 微生物学 肠炎沙门氏菌 生物 食品科学 分离(微生物学) 细菌 化学 大肠杆菌 遗传学 生物化学 基因
作者
Chenxi Huang,Shi Jian-chun,Wenjuan Ma,Li Zhi,Jia Wang,Jinquan Li,Xiaohong Wang
出处
期刊:Food Research International [Elsevier]
卷期号:111: 631-641 被引量:110
标识
DOI:10.1016/j.foodres.2018.05.071
摘要

Application of bacteriophages to eliminate foodborne pathogens in food matrices is an emerging research field. In this study, a promising phage candidate specific for Salmonella strains was screened and its ability to decrease Salmonella counts in some food, such as milk, sausage, and lettuce, was investigated. A total of 58 Salmonella phages were isolated from a wastewater treatment plant, sewage near a river, farm ditch near a lake, and poultry house. Among them, phages LPST10, LPST18, and LPST23 were highly efficient in infecting Salmonella Typhimurium ATCC 14028. In particular, phage LPST10 could infect all the tested Salmonella Typhimurium and Salmonella Enteritidis strains with high efficiency. Bacterial challenge tests revealed that phage LPST10 and its combination with phages LPST18 and LPST23 could consistently inhibit the growth of multiple strains. Phage LPST10 presented a lysis time of about 50 min with a burst size of 101 PFU/CFU, exhibited two distinct phases in the one-step growth curve, and was stable at a pH range of 3–13 that corresponds to the pH of most of the foods (pH 3.5–7.5) and at temperatures between 30 °C and 60 °C. Transmission electron microscopy demonstrated that phage LPST10 belongs to the Siphoviridae family, with an icosahedral head with a diameter of 83.26 nm and tail length and width of approximately 144.89 nm and 10.9 nm, respectively. A significant decrease in the bacterial counts (0.92–5.12 log10 CFU/sample) and an increase in phage titers (0–2.96 log10 PFU/sample) were observed in different food matrices tested. These results demonstrated that phage LPST10 is a promising candidate for controlling Salmonella contamination in foods owing to its safety and effectiveness.
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