心肌保护
缺血
医学
心功能曲线
心脏病学
再灌注损伤
线粒体融合
内科学
心肌梗塞
线粒体通透性转换孔
线粒体分裂
线粒体
药理学
麻醉
细胞凋亡
生物
线粒体DNA
心力衰竭
程序性细胞死亡
基因
细胞生物学
生物化学
作者
Chayodom Maneechote,Siripong Palee,Sasiwan Kerdphoo,Thidarat Jaiwongkam,Nipon Chattipakorn,Nipon Chattipakorn
出处
期刊:Clinical Science
[Portland Press]
日期:2019-02-01
卷期号:133 (3): 497-513
被引量:70
摘要
Abstract An uncontrolled balance of mitochondrial dynamics has been shown to contribute to cardiac dysfunction during ischemia/reperfusion (I/R) injury. Although inhibition of mitochondrial fission could ameliorate cardiac dysfunction, modulation of mitochondrial fusion by giving a fusion promoter at different time-points during cardiac I/R injury has never been investigated. We hypothesized that giving of a mitochondrial fusion promoter at different time-points exerts cardioprotection with different levels of efficacy in rats with cardiac I/R injury. Forty male Wistar rats were subjected to a 30-min ischemia by coronary occlusion, followed by a 120-min reperfusion. The rats were then randomly divided into control and three treated groups: pre-ischemia, during-ischemia, and onset of reperfusion. A pharmacological mitochondrial fusion promoter-M1 (2 mg/kg) was used for intervention. Reduced mitochondrial fusion protein was observed after cardiac I/R injury. M1 administered prior to ischemia exerted the highest level of cardioprotection by improving both cardiac mitochondrial function and dynamics regulation, attenuating incidence of arrhythmia, reducing infarct size and cardiac apoptosis, which led to the preservation of cardiac function and decreased mortality. M1 given during ischemia and on the onset of reperfusion also exerted cardioprotection, but with a lower efficacy than when given at the pre-ischemia time-point. Attenuating a reduction in mitochondrial fusion proteins during myocardial ischemia and at the onset of reperfusion exerted cardioprotection by attenuating mitochondrial dysfunction and dynamic imbalance, thus reducing infarct size and improving cardiac function. These findings indicate that it could be a promising intervention with the potential to afford cardioprotection in the clinical setting of acute myocardial infarction.
科研通智能强力驱动
Strongly Powered by AbleSci AI