细胞凋亡
机制(生物学)
细胞周期检查点
胡桃醌
子宫内膜癌
癌症研究
细胞周期
化学
细胞生物学
程序性细胞死亡
癌症
内科学
生物
医学
生物化学
哲学
认识论
作者
Yuanyuan Zhang,Fan Zhang,Ying-Shuo Zhang,Kiran Thakur,Jian‐Guo Zhang,Yun Liu,Huan Kan,Zhao‐Jun Wei
标识
DOI:10.1021/acs.jafc.9b02759
摘要
The molecular mechanism of Juglone-induced cell cycle arrest and apoptosis in human endometrial cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 μM, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein (p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, CHK1, and cyclin A. The expression of Bcl-2 and Bcl-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural anticancer agent.
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