Antibacterial Activity of Lactobacillus (LB) Strains Isolated from Goat Milk against ESBL Producing E. coli Causing Wound Infections

Lactobacillus (LB) species are well known probiotics with beneficial effects to human health. Their antimicrobial activity is one of the most important probiotic characteristics. Wound infections are the most frequent bacterial infections encountered in community settings. Extended spectrum β-lactamases (ESBLs) are enzymes produced by pathogenic bacteria that enable such pathogenic bacteria as multi drug resistant. Treatment of infections by these drug resistant bacteria require newer anti microbialsthat are highly expensive and possibility of many side effects. Microbial products consideredas the best source for therapeutic agents worldwide. Lactobacillus species were isolated from 2 days fermented goat Milk. Theantimicrobial activity of cell-free supernatant and partially purified bacteriocin was determined by well diffusion method and equal mixed culture method. All isolated ESBL were processed and identified as per the CLSI guide lines. The organism was identified by GN ID and AST 280 of Vitec-II campact system and AES (Advance extended spectrum) tests. The current study showed that ESBL E. coli resistant to Cefazoline (96.6%), Ceftazidime (43.3%), Ceftriaxone (3.3%), Ciprofloxacin (76.6%), Meropenem (0%), Tobramycin (43.3%), Cefotaxime (93.3%), Ceftazidime + cluvalonic acid (3.3%), Cefotaxime + cluvalonic acid (3.3%), Cefperodoxime (96.6%), Piperacillin + Tazobactam (0%), Ampicillin (100%). Lactobacillus species exhibited an antibacterial effect on a narrow range of ESBL strains. However invitro effect is better than large number broad-spectrum antibiotics such: third-generation cephalosporins