Effects of He–Ne laser and argon laser irradiation on growth, germination, and physico-biochemical characteristics of wheat seeds (Triticumaestivum L.)

Wheat seeds (Triticumaestivum L.) were exposed to two different types of lasers, a He–Ne laser (633 nm) and an argon laser (514.5 nm), to study their effects on growth, germination, and physiological and biochemical characteristics such as the amounts of chlorophyll a, chlorophyll b, and carotenoids. A significant increase in the growth characteristics was monitored and studied keeping in view the length as well as fresh and dry weight of shoots and roots. Similarly, plant pigment contents such as chlorophyll a, chlorophyll b, and carotenoids were significantly changed. These significant alterations led to an increase in growth and germination compared with unexposed (control) wheat seeds. The experimental results of this in vitro study clearly demonstrate that different lasers like He–Ne laser and argon laser at low power significantly enhance the growth and germination of wheat seeds (Triticumaestivum L.) by positively influencing its physiological and biochemical characteristics.