Reaction Kinetics and Stoichiometry

Biochemical reaction kinetics for enzyme and cell (including microbe, plant, and animal cells) are reviewed. Fundamental concept and/or background for the kinetics model construction and the parameter determination of model using experimental data are assessed. Factors affecting the kinetics such as inhibitor, temperature, pH, product, and substrate are also discussed. Stoichiometric consideration of cell reaction is made in order to clarify the elemental balance of the process. Yields of biochemical reaction process occurring in cells are defined in terms of various variables such as biomass, product, heat of reaction as a function of substrate, oxygen, adenosine triphosphate (ATP), etc. Empirical, but universal relationships are suggested in that the elemental composition of microbial cell is similar among various microbial species, and that the cell mass yield referred to ATP is quite similar independently of microbial species and of utilized substrates.