血管生成
微血管病
脐静脉
糖尿病
医学
小RNA
内皮功能障碍
内科学
人脐静脉内皮细胞
内皮干细胞
细胞凋亡
内分泌学
糖尿病血管病
2型糖尿病
癌症研究
生物
体外
基因
生物化学
作者
Shruti Rawal,Pujika Emani Munasinghe,Amol Shindikar,Jono Paulin,Vicky A. Cameron,Patrick Manning,Michael Williams,Gregory T. Jones,Richard W. Bunton,Ivor F. Galvin,Rajesh Katare
出处
期刊:Cardiovascular Research
[Oxford University Press]
日期:2016-11-16
卷期号:113 (1): 90-101
被引量:81
摘要
Microangiopathy due to endothelial dysfunction is a major contributing factor to the development of diabetes-induced cardiovascular disease (CVD). Dysregulation of endothelial-specific microRNAs (miRs) is correlated with impaired angiogenesis and cell survival. We investigated the profile of two angiomiRs, miR-126, and miR-132, in the plasma of type 2 diabetic individuals without any known history of CVD as well as in the cardiac tissues collected from diabetics undergoing cardiac surgery. The presence of diabetes alone significantly decreased both angiomiRs in the plasma and the myocardium. The down-regulation of angiomiRs was also associated with reduced capillaries and arterioles and increased endothelial cell apoptosis, the hallmark of microangiopathy. Importantly, a time course study in a type 2 diabetic mouse model confirmed that the down-regulation of angiomiRs preceded endothelial apoptosis as well as alterations in the density of the microvasculature. Finally, therapeutic overexpression of both angiomiRs in diabetic aortic rings and human umbilical vein endothelial cells exposed to high glucose (HG) abrogated the deleterious effects of diabetes and HG on cell survival and proliferation and restored their angiogenic potential. These novel findings demonstrate that the down-regulation of angiomiRs is a major underlying mechanism for the development of microangiopathy in diabetic hearts. Therefore, therapeutic restoration of angiomiRs could become a potential approach to combat the cardiovascular complications of diabetes.
科研通智能强力驱动
Strongly Powered by AbleSci AI