抗辐射性
自噬
缺氧(环境)
活性氧
程序性细胞死亡
线粒体ROS
线粒体
生物
癌症研究
细胞生物学
癌细胞
克隆形成试验
A549电池
细胞
化学
癌症
细胞凋亡
细胞培养
生物化学
氧气
有机化学
遗传学
作者
Xiaohong Chen,Ping Wang,Fei Guo,Xiangdong Wang,Juan Wang,XU Jin-ping,Dexiao Yuan,Jianghong Zhang,Chunlin Shao
标识
DOI:10.1080/09553002.2017.1325025
摘要
Tumor resistance towards radiation has been a big obstacle in the poor prognosis of lung cancer. It has been reported that hypoxia and autophagy partly contribute to this resistance. However, there is controversy over whether autophagy plays a positive role in cancer therapy or not. We aim to find out the specific mechanism of radiation resistance.A549 cells were treated with conditioned medium (CM) under 12 h hypoxia or normoxia before irradiation, followed by the measurement of clonogenic survival, reactive oxygen species (ROS), signal of mitochondria and autophagy flux. In some experiments, the A549 cells were respectively transfected with LC3 small interfering RNA (siRNA), or treated with Earle's Balanced Salt Solution (EBSS).We found that hypoxia enhanced cell radioresistance by increasing the induction of autophagy. And after hypoxia stress, the number of mitochondria was reduced but the cellular ROS level was enhanced. It was significant that autophagy may enhance cell radioresistance by reducing ROS during hypoxic treatment.We elucidated the possible mechanisms of autophagy in regulating cancer cell death or survival. These results supply a new opinion about the intrinsic factor of radioresistance of hypoxia tumors.
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