Effects of temperature and diet on the reproduction of the rockworm Marphysa sanguinea (Montagu, 1815)

Event Abstract Back to Event Effects of temperature and diet on the reproduction of the rockworm Marphysa sanguinea (Montagu, 1815) Marcela França1, Daniela Machado1, Catarina M. Anjos1, Carmen Pedro1, Mafalda Catarino1, Teresa Baptista1, Susana M. Ferreira1, 2, Sílvia C. Gonçalves1, 3, Pedro Fidalgo E Costa4, José Lino V. Costa5 and Ana Pombo1* 1 MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal, Portugal 2 CFE – Centre for Functional Ecology, Department of Life Science, University of Coimbra, Apartado 3046, 3001-401 Coimbra, Portugal, Portugal 3 MARE – Marine and Environmental Sciences Centre, Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, 3004-517 Coimbra, Portugal, Portugal 4 MARE– Marine and Environmental Sciences Centre, Laboratório Marítimo da Guia, Av. N. Sra. do Cabo, 939, 750-374, Portugal, Portugal 5 MARE – Marine and Environmental Sciences Centre, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal, Portugal The increasing commercial importance of polychaetes as fishing bait and feed source in aquaculture and, consequently, their massive harvesting, are causing disturbances in the benthic communities and in the ecosystems. For these reasons the increase of polychaete production in aquaculture is important, to avoid the depletion of a natural resource and minimise the negative impacts in the environment. The development and optimisation of rearing techniques for Marphysa sanguinea at industrial scale is crucial and one of the key steps for the success of the growing polychaetes. This production requires reproduction and nutrition knowledge. In order to develop rearing techniques for M. sanguinea, the influence of temperature and diet on the reproduction were studied. Adults of rockworm M. sanguinea (n=144) with 1.8 ± 0.2 g weight were stocked in triplicate in recirculating aquaculture systems (A, B, C, D) with three aquaria (60 L) each; the sex ratio was 1:1. The aquaria were provided with a 10 cm depth sand-layer. Two different temperatures (18 ± 1 ºC and 22 ± 1 ºC) and two diets (60 % protein, 2 % Lipids – 60P2L and 46 % protein and 18 % lipids – 46P18L) were tested during two and a half months. Organisms were fed ad libitum daily. The four different conditions in the systems were: 18 ± 1ºC and 60P2L (System A); 18 ± 1ºC and 46P18L (System B); 22 ± 1ºC and 60P2L (System C); 22 ± 1 ºC and 46P18L (System D). After this period worms were induced to spawn by thermal shock (4 ºC). A month after the thermal shock a sample of the living organisms present in all aquaria was obtained, following a gentle sieving to collect them, and were afterwards counted and measured. With this procedure, the density in the systems was achieved. Results showed that the system C presented a significant higher density (533035 ind.m-2) than the other systems: 332517 ind.m-2 in the system A; 350150 ind.m-2 in the system B and 16625 ind.m-2 in the system D. Furthermore, it should be noted that juveniles obtained in the system C presented a significantly higher length (711.2 ± 58.9 µm) than the juveniles coming from the other systems (476.5 ± 74 µm, 465.2 ± 66 µm, 334.74 ± 90 µm in the systems A, B and D respectively). The higher temperature 22 ± 1 ºC combined with a high protein diet (System C) seems to promote better results in juvenile production. This highlights the significant influence of water temperature on the reproductive cycle of this species with a diet with high content of protein, however, the system A and B densities can be also considered productive. Nevertheless, taking into account the higher price of protein as diet component, a cheaper alternative may be the utilization of a more balanced diet, such as the diet 48P18L, combined with the lower temperature (18ºC) similarly to what was used in the system B. This conditions can cause less impact in the juvenile’s production, in terms of density, than with the conditions of the System D. The high number of juveniles recorded in this study with broodstock of M. sanguinea fed with extruded formulated diets suggests that this species may be suitable for reproduction and growing in indoor recirculating aquaculture systems of commercial exploitation. Acknowledgements This study had the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE, and the PROMAR Program through the project 31-03-05-FEP-42: LIVE BAIT - Annelid polychaetes as live bait in Portugal: harvesting, import and rearing management. Keywords: polychaetes reproduction, polychaetes nutrition, sea bait aquaculture, worms rearing, Recirculating aquaculture systems Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Aquaculture Citation: França M, Machado D, Anjos CM, Pedro C, Catarino M, Baptista T, Ferreira SM, Gonçalves SC, Fidalgo E Costa P, Costa JV and Pombo A (2016). Effects of temperature and diet on the reproduction of the rockworm Marphysa sanguinea (Montagu, 1815). Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Apr 2016; Published Online: 13 Jul 2016. * Correspondence: Prof. Ana Pombo, MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal, Peniche, Portugal, ana.pombo@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marcela França Daniela Machado Catarina M Anjos Carmen Pedro Mafalda Catarino Teresa Baptista Susana M Ferreira Sílvia C Gonçalves Pedro Fidalgo E Costa José Lino V Costa Ana Pombo Google Marcela França Daniela Machado Catarina M Anjos Carmen Pedro Mafalda Catarino Teresa Baptista Susana M Ferreira Sílvia C Gonçalves Pedro Fidalgo E Costa José Lino V Costa Ana Pombo Google Scholar Marcela França Daniela Machado Catarina M Anjos Carmen Pedro Mafalda Catarino Teresa Baptista Susana M Ferreira Sílvia C Gonçalves Pedro Fidalgo E Costa José Lino V Costa Ana Pombo PubMed Marcela França Daniela Machado Catarina M Anjos Carmen Pedro Mafalda Catarino Teresa Baptista Susana M Ferreira Sílvia C Gonçalves Pedro Fidalgo E Costa José Lino V Costa Ana Pombo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.