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Rationally Tailored Mesoporous Hosts for Optimal Protein Encapsulation

介孔材料 化学 封装(网络) 纳米技术 催化作用 材料科学 计算机科学 生物化学 计算机网络
作者
Fanrui Sha,Haomiao Xie,Florencia A. Son,Kevin S. Kim,Wei Gong,Shengyi Su,Kaikai Ma,Xiaoliang Wang,Xingjie Wang,Omar K. Farha
出处
期刊:Journal of the American Chemical Society [American Chemical Society]
卷期号:145 (30): 16383-16390 被引量:17
标识
DOI:10.1021/jacs.3c01989
摘要

Proteins play important roles in the therapeutic, medical diagnostic, and chemical catalysis industries. However, their potential is often limited by their fragile and dynamic nature outside cellular environments. The encapsulation of proteins in solid materials has been widely pursued as a route to enhance their stability and ease of handling. Nevertheless, the experimental investigation of protein interactions with rationally designed synthetic hosts still represents an area in need of improvement. In this work, we leveraged the tunability and crystallinity of metal-organic frameworks (MOFs) and developed a series of crystallographically defined protein hosts with varying chemical properties. Through systematic studies, we identified the dominating mechanisms for protein encapsulation and developed a host material with well-tailored properties to effectively encapsulate the protein ubiquitin. Specifically, in our mesoporous hosts, we found that ubiquitin encapsulation is thermodynamically favored. A more hydrophilic encapsulation environment with favorable electrostatic interactions induces enthalpically favored ubiquitin-MOF interactions, and a higher pH condition reduces the intraparticle diffusion barrier, both leading to a higher protein loading. Our findings provide a fundamental understanding of host-guest interactions between proteins and solid matrices and offer new insights to guide the design of future protein host materials to achieve optimal protein loading. The MOF modification technique used in this work also demonstrates a facile method to develop materials easily customizable for encapsulating proteins with different surface properties.
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