Mahuang Xixin Fuzi decoction protects the BALB/c-nude mice infected with influenza A virus by reducing inflammatory cytokines storm and weakly regulating SIgA immune response

免疫学 免疫系统 脾脏 支气管肺泡灌洗 肿瘤坏死因子α 抗体 趋化因子 医学 生物 内科学
作者
Meiyi Liu,Jia Yang,Shensi Qian,Zhuyun Sun,Y. Jin,Xiaoyun Liu,Dongxue Ye,Rong Rong,Yong Yang
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:304: 116070-116070 被引量:7
标识
DOI:10.1016/j.jep.2022.116070
摘要

Mahuang Xixin Fuzi Decoction (MXF), as a classical prescription of traditional Chinese medicine (TCM), has been used to treat the immunocompromised individuals infected with influenza A virus (IAV).The study aims to explore the regulatory of MXF on inflammation and secretory immunoglobulin A (SIgA) antibodies immune response in BALB/c-nude mice infected with IAV.The BALB/c-nude mice were infected with IAV, then different dosages of MXF were orally administrated to the mice. The weight, rectal temperature, spontaneous activity, spleen index, lung index, pathological changes of lung tissues, and the relative mRNA expression level of H1N1 M gene were measured for the purpose of valuing the antiviral effect of MXF. The expression levels of cytokines in lungs and immunoglobulin A (IgA) in serum of BALB/c-nude mice were determined with Cytometric Bead Array System (CBA). SIgA in bronchoalveolar lavage fluids (BALF) was detected with Enzyme-linked Immunosorbent Assay (ELISA). The mRNA and protein expression levels of B cell activating factor (BAFF), chemokine receptors 10 (CCR10), and polymeric immunoglobulin receptor (pIgR) in the lung tissues, which are related to the secretion of SIgA, were determined by using RT-PCR and Western blot.MXF could alleviate the clinical features and reduce the severity of viral lung lesions, including improving the body weight, rectal temperature and spontaneous activity of nude mice infected with IAV, increasing spleen index, decreasing lung index, alleviating pathological damage, and decreasing the relative expression level of H1N1 M gene. Levels of pro-inflammatory cytokines, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-12p70 (IL-12p70), and interleukin-17A (IL-17A) were also significantly decreased after treatment with MXF. Interferon-γ (IFN-γ), an antiviral cytokine, was significantly up-regulated in high dose MXF (3.12 g/kg) group. Moreover, after MXF treatment, the expressions of SIgA in BALF and IgA in serum were both at relatively low levels. And the mRNA and protein expressions of BAFF, CCR10, and pIgR were significantly decreased after treatment with MXF.MXF has obviously protective effects on BALB/c-nude mice infected with IAV by inhibiting virus replication, calming inflammatory cytokine storm, and regulating SIgA immune response weakly.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI2S应助科研通管家采纳,获得30
刚刚
英姑应助科研通管家采纳,获得10
刚刚
Akim应助科研通管家采纳,获得20
刚刚
sota完成签到,获得积分10
刚刚
无花果应助科研通管家采纳,获得10
刚刚
刚刚
刚刚
Ava应助科研通管家采纳,获得10
刚刚
爆米花应助科研通管家采纳,获得10
1秒前
SciGPT应助科研通管家采纳,获得10
1秒前
1秒前
田様应助科研通管家采纳,获得10
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
1秒前
英俊的铭应助科研通管家采纳,获得10
1秒前
灰色与青完成签到,获得积分10
2秒前
bkagyin应助ylw采纳,获得10
2秒前
星辰大海应助kcmat采纳,获得10
3秒前
4秒前
qq完成签到,获得积分10
4秒前
4秒前
4秒前
5秒前
上官若男应助无心的土豆采纳,获得10
6秒前
honeylaker发布了新的文献求助10
6秒前
7秒前
callmecjh发布了新的文献求助10
7秒前
9月有书读发布了新的文献求助10
8秒前
9秒前
英俊的铭应助忧虑的尔容采纳,获得10
10秒前
糖糖完成签到,获得积分10
10秒前
Enoch发布了新的文献求助10
11秒前
科目三应助等待八宝粥采纳,获得10
11秒前
11秒前
亨利公爵完成签到,获得积分10
12秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds第二卷 1200
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038657
求助须知:如何正确求助?哪些是违规求助? 3576306
关于积分的说明 11375198
捐赠科研通 3306108
什么是DOI,文献DOI怎么找? 1819379
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066