Image-based cell sorting using focused travelling surface acoustic waves

分类 单元格排序 人口 微流控 细胞仪 吞吐量 分类 生物系统 纳米技术 材料科学 计算机科学 细胞 生物医学工程 化学 生物 工程类 算法 人口学 无线 社会学 电信 生物化学 情报检索
作者
Ahsan Ahmad Nawaz,Despina Soteriou,Catherine K. Xu,Ruchi Goswami,Maik Herbig,Jochen Guck,Salvatore Girardo
出处
期刊:Lab on a Chip [Royal Society of Chemistry]
卷期号:23 (2): 372-387 被引量:16
标识
DOI:10.1039/d2lc00636g
摘要

Sorting cells is an essential primary step in many biological and clinical applications such as high-throughput drug screening, cancer research and cell transplantation. Cell sorting based on their mechanical properties has long been considered as a promising label-free biomarker that could revolutionize the isolation of cells from heterogeneous populations. Recent advances in microfluidic image-based cell analysis combined with subsequent label-free sorting by on-chip actuators demonstrated the possibility of sorting cells based on their physical properties. However, the high purity of sorting is achieved at the expense of a sorting rate that lags behind the analysis throughput. Furthermore, stable and reliable system operation is an important feature in enabling the sorting of small cell fractions from a concentrated heterogeneous population. Here, we present a label-free cell sorting method, based on the use of focused travelling surface acoustic wave (FTSAW) in combination with real-time deformability cytometry (RT-DC). We demonstrate the flexibility and applicability of the method by sorting distinct blood cell types, cell lines and particles based on different physical parameters. Finally, we present a new strategy to sort cells based on their mechanical properties. Our system enables the sorting of up to 400 particles per s. Sorting is therefore possible at high cell concentrations (up to 36 million per ml) while retaining high purity (>92%) for cells with diverse sizes and mechanical properties moving in a highly viscous buffer. Sorting of small cell fraction from a heterogeneous population prepared by processing of small sample volume (10 μl) is also possible and here demonstrated by the 667-fold enrichment of white blood cells (WBCs) from raw diluted whole blood in a continuous 10-hour sorting experiment. The real-time analysis of multiple parameters together with the high sensitivity and high-throughput of our method thus enables new biological and therapeutic applications in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
搜集达人应助NMZN采纳,获得10
1秒前
含蓄绿兰发布了新的文献求助10
1秒前
HHH完成签到,获得积分10
2秒前
FashionBoy应助毕春宇采纳,获得10
2秒前
桐桐应助嗯呐采纳,获得10
2秒前
2秒前
UltraYuan完成签到,获得积分20
3秒前
852应助BING采纳,获得10
3秒前
研友_VZG7GZ应助科研通管家采纳,获得10
3秒前
3秒前
JamesPei应助科研通管家采纳,获得10
3秒前
CipherSage应助科研通管家采纳,获得10
3秒前
田様应助科研通管家采纳,获得10
3秒前
情怀应助科研通管家采纳,获得10
3秒前
丘比特应助秋水揽星河采纳,获得10
3秒前
CrysField完成签到,获得积分10
3秒前
无花果应助科研通管家采纳,获得10
4秒前
4秒前
4秒前
FashionBoy应助科研通管家采纳,获得10
4秒前
4秒前
4秒前
kelly完成签到,获得积分20
4秒前
FashionBoy应助科研通管家采纳,获得10
4秒前
大模型应助李蹦跶采纳,获得10
5秒前
5秒前
脑洞疼应助罗劲松采纳,获得10
5秒前
自然发布了新的文献求助10
5秒前
852应助chyse采纳,获得10
6秒前
7秒前
orixero应助飞行中的鱼采纳,获得10
7秒前
niuniu完成签到,获得积分10
7秒前
7秒前
牛贝贝完成签到,获得积分10
7秒前
zhezhe发布了新的文献求助10
8秒前
乐乐应助彩色新筠采纳,获得10
8秒前
able完成签到 ,获得积分10
8秒前
马敬丽发布了新的文献求助10
9秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971091
求助须知:如何正确求助?哪些是违规求助? 3515797
关于积分的说明 11179488
捐赠科研通 3250872
什么是DOI,文献DOI怎么找? 1795536
邀请新用户注册赠送积分活动 875891
科研通“疑难数据库(出版商)”最低求助积分说明 805207