Image-based cell sorting using focused travelling surface acoustic waves

分类 单元格排序 人口 微流控 细胞仪 吞吐量 分类 生物系统 纳米技术 材料科学 计算机科学 细胞 生物医学工程 化学 生物 工程类 算法 人口学 无线 社会学 电信 生物化学 情报检索
作者
Ahsan Ahmad Nawaz,Despina Soteriou,Catherine K. Xu,Ruchi Goswami,Maik Herbig,Jochen Guck,Salvatore Girardo
出处
期刊:Lab on a Chip [Royal Society of Chemistry]
卷期号:23 (2): 372-387 被引量:16
标识
DOI:10.1039/d2lc00636g
摘要

Sorting cells is an essential primary step in many biological and clinical applications such as high-throughput drug screening, cancer research and cell transplantation. Cell sorting based on their mechanical properties has long been considered as a promising label-free biomarker that could revolutionize the isolation of cells from heterogeneous populations. Recent advances in microfluidic image-based cell analysis combined with subsequent label-free sorting by on-chip actuators demonstrated the possibility of sorting cells based on their physical properties. However, the high purity of sorting is achieved at the expense of a sorting rate that lags behind the analysis throughput. Furthermore, stable and reliable system operation is an important feature in enabling the sorting of small cell fractions from a concentrated heterogeneous population. Here, we present a label-free cell sorting method, based on the use of focused travelling surface acoustic wave (FTSAW) in combination with real-time deformability cytometry (RT-DC). We demonstrate the flexibility and applicability of the method by sorting distinct blood cell types, cell lines and particles based on different physical parameters. Finally, we present a new strategy to sort cells based on their mechanical properties. Our system enables the sorting of up to 400 particles per s. Sorting is therefore possible at high cell concentrations (up to 36 million per ml) while retaining high purity (>92%) for cells with diverse sizes and mechanical properties moving in a highly viscous buffer. Sorting of small cell fraction from a heterogeneous population prepared by processing of small sample volume (10 μl) is also possible and here demonstrated by the 667-fold enrichment of white blood cells (WBCs) from raw diluted whole blood in a continuous 10-hour sorting experiment. The real-time analysis of multiple parameters together with the high sensitivity and high-throughput of our method thus enables new biological and therapeutic applications in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
他也蓝发布了新的文献求助10
1秒前
芋泥面包发布了新的文献求助10
1秒前
liyb完成签到,获得积分10
2秒前
绵羊座鸭梨完成签到 ,获得积分10
2秒前
orixero应助drxiao采纳,获得30
3秒前
千冬完成签到,获得积分10
6秒前
李凭中国弹箜篌完成签到,获得积分10
6秒前
8秒前
浪客完成签到 ,获得积分10
8秒前
韩soso完成签到,获得积分10
9秒前
10秒前
完美的从波完成签到,获得积分10
10秒前
SilvanYang完成签到,获得积分10
11秒前
张建文完成签到,获得积分10
12秒前
13秒前
14秒前
枫泾发布了新的文献求助10
14秒前
14秒前
JamesPei应助xiaohuangya采纳,获得10
15秒前
15秒前
妖孽发布了新的文献求助10
16秒前
16秒前
田様应助June采纳,获得10
16秒前
17秒前
完美大神完成签到 ,获得积分10
18秒前
18秒前
hunbaekkkkk发布了新的文献求助10
19秒前
19秒前
syuen发布了新的文献求助10
20秒前
20秒前
20秒前
20秒前
啊薇儿发布了新的文献求助10
21秒前
21秒前
22秒前
23秒前
方方发布了新的文献求助30
23秒前
ma完成签到,获得积分20
24秒前
深海敢敢完成签到 ,获得积分20
25秒前
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976267
求助须知:如何正确求助?哪些是违规求助? 3520472
关于积分的说明 11203425
捐赠科研通 3257089
什么是DOI,文献DOI怎么找? 1798589
邀请新用户注册赠送积分活动 877785
科研通“疑难数据库(出版商)”最低求助积分说明 806523