Label‐Free Multiphoton Microscopy for Diagnosis of Psoriasis

ABSTRACT Psoriasis is a global problem that significantly affects patients both physically and mentally. Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod‐induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross‐linking gap of collagen fibers, as well as an increase in cross‐linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. Overall, our study presents a rapid diagnostic method for psoriasis using MPM.