抗坏血酸
化学
过氧化氢
过氧化物酶
煅烧
组合化学
检出限
催化作用
无机化学
纳米技术
核化学
色谱法
酶
生物化学
材料科学
食品科学
作者
Wenzhi Zhang,Yuxin Lan,Dong-Feng Chai,Jun Liu,Guohua Dong,Dongxuan Guo
标识
DOI:10.1016/j.molstruc.2024.137507
摘要
The development of artificial nanostructured enzymes has garnered significant interest in the field of nanobiotechnology due to the distinct advantages. In this work, CeO2/Co3O4 hollow nanocubes (named as CeO2/Co3O4 HNs) with exceptional peroxidase-like activity have been prepared employing ion exchange and subsequent calcination process. In the presence of hydrogen peroxide (H2O2), the CeO2/Co3O4 HNs display high peroxidase-like activity, catalyzing the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and resulting in a color change from transparent to blue. The oxidized TMB (ox-TMB) is subsequently reduced to TMB due to the reducibility of ascorbic acid (AA), restoring the original color of solution. Consequently, a straightforward and efficient "On-Off" colorimetric sensor has been established for the accurate detection of H2O2 and AA, with detection limits (LOD) of 3.56 and 0.179 μM, respectively. In summary, the prepared colorimetric sensor offers numerous advantages, including ease of operation, cost-effectiveness, rapid response, and effective visual monitoring, which holds substantial promise for applications in detection of hydrogen peroxide and ascorbic acid.
科研通智能强力驱动
Strongly Powered by AbleSci AI