尿
检出限
金标准(测试)
微生物学
色谱法
电动现象
抗生素
抗菌剂
医学
化学
生物
生物化学
内科学
物理化学
作者
Siqi Wei,Qing Tang,Xiumei Hu,Wei Ouyang,Huaze Shao,Jincheng Li,Hong Yan,Yue Chen,Lihong Liu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-01-12
卷期号:9 (1): 325-336
被引量:1
标识
DOI:10.1021/acssensors.3c02014
摘要
Rapid prescribing of the right antibiotic is the key to treat infectious diseases and decelerate the challenge of bacterial antibiotic resistance. Herein, by targeting the 16S rRNA of bacteria, we developed a cation dye-triggered electrokinetic gold nanoparticle (AuNP) agglutination (CD-TEAA) method, which is rapid, visual, ultrasensitive, culture-independent, and low in cost. The limit of detection (LOD) is as low as 1 CFU mL–1 Escherichia coli. The infection identifications of aseptic fluid samples (n = 11) and urine samples with a clinically suspected urinary tract infection (UTI, n = 78) were accomplished within 50 and 30 min for each sample, respectively. The antimicrobial susceptibility testing (AST) of UTI urine samples was achieved within 2.5 h. In ROC analysis of urine, the sensitivity and specificity were 100 and 96% for infection identification, and 100 and 98% for AST, respectively. Moreover, the overall cost of materials for each test is about US$0.69. Therefore, the CD-TEAA method is a superior approach to existing, time-consuming, and expensive methods, especially in less developed areas.
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