亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Mechanism of Action and Design of Potent Antibacterial Block Copolymer Nanoparticles

化学 共聚物 抗菌活性 分散性 纳米纤维 结晶度 纳米颗粒 化学工程 抗菌剂 纳米技术 高分子化学 有机化学 生物化学 细菌 结晶学 聚合物 材料科学 抗生素 生物 工程类 遗传学
作者
Hayley C. Parkin,Steven T. G. Street,Brent E. Gowen,Luiz H. Da-Silva-Correa,Rebecca Hof,Heather L. Buckley,Ian Manners
出处
期刊:Journal of the American Chemical Society [American Chemical Society]
卷期号:146 (8): 5128-5141 被引量:3
标识
DOI:10.1021/jacs.3c09033
摘要

Self-assembled polymer nanoparticles are promising antibacterials, with nonspherical morphologies of particular interest as recent work has demonstrated enhanced antibacterial activity relative to their spherical counterparts. However, the reasons for this enhancement are currently unclear. We have performed a multifaceted analysis of the antibacterial mechanism of action of 1D nanofibers relative to nanospheres by the use of flow cytometry, high-resolution microscopy, and evaluations of the antibacterial activity of pristine and tetracycline-loaded nanoparticles. Low-length dispersity, fluorescent diblock copolymer nanofibers with a crystalline poly(fluorenetrimethylenecarbonate) (PFTMC) core (length = 104 and 472 nm, height = 7 nm, width = 10–13 nm) and a partially protonated poly(dimethylaminoethyl methacrylate) (PDMAEMA) corona (length = 12 nm) were prepared via seeded growth living crystallization-driven self-assembly. Their behavior was compared to that of analogous nanospheres containing an amorphous PFTMC core (diameter of 12 nm). While all nanoparticles were uptaken into Escherichia coli W3110, crystalline-core nanofibers were observed to cause significant bacterial damage. Drug loading studies indicated that while all nanoparticle antibacterial activity was enhanced in combination with tetracycline, the enhancement was especially prominent when small nanoparticles (ca. 15–25 nm) were employed. Therefore, the identified differences in the mechanism of action and the demonstrated consequences for nanoparticle size and morphology control may be exploited for the future design of potent antibacterial agents for overcoming antibacterial resistance. This study also reinforces the requirement of morphological control over polymer nanoparticles for biomedical applications, as differences in activity are observed depending on their size, shape, and core-crystallinity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
asdfqaz完成签到,获得积分10
1分钟前
Georgechan完成签到,获得积分10
1分钟前
田様应助fff采纳,获得10
2分钟前
2分钟前
3分钟前
fff发布了新的文献求助10
3分钟前
张小汉完成签到,获得积分10
4分钟前
CUN完成签到,获得积分10
4分钟前
Owen应助fff采纳,获得10
4分钟前
4分钟前
NexusExplorer应助Yogurt采纳,获得10
4分钟前
冬去春来完成签到 ,获得积分10
5分钟前
5分钟前
6分钟前
fff发布了新的文献求助10
6分钟前
在水一方应助fff采纳,获得10
6分钟前
月军完成签到,获得积分10
6分钟前
6分钟前
金钰贝儿完成签到,获得积分10
7分钟前
8分钟前
9分钟前
fff发布了新的文献求助10
9分钟前
赘婿应助fff采纳,获得10
9分钟前
巅峰囚冰完成签到,获得积分10
9分钟前
10分钟前
10分钟前
zsmj23完成签到 ,获得积分0
10分钟前
11分钟前
fff发布了新的文献求助10
11分钟前
lixiaoya完成签到,获得积分10
11分钟前
NexusExplorer应助fff采纳,获得10
11分钟前
莓啤汽完成签到 ,获得积分10
11分钟前
13分钟前
fff发布了新的文献求助10
13分钟前
vitamin完成签到 ,获得积分10
14分钟前
dandan完成签到,获得积分10
14分钟前
M先生完成签到,获得积分10
14分钟前
14分钟前
陈东生发布了新的文献求助30
14分钟前
15分钟前
高分求助中
Sustainability in Tides Chemistry 1500
Handbook of the Mammals of the World – Volume 3: Primates 805
拟南芥模式识别受体参与调控抗病蛋白介导的ETI免疫反应的机制研究 550
Gerard de Lairesse : an artist between stage and studio 500
Digging and Dealing in Eighteenth-Century Rome 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Manual of Sewer Condition Classification 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3068122
求助须知:如何正确求助?哪些是违规求助? 2722122
关于积分的说明 7476029
捐赠科研通 2369115
什么是DOI,文献DOI怎么找? 1256205
科研通“疑难数据库(出版商)”最低求助积分说明 609490
版权声明 596826